Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11516] to NAF1
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Product nameAnti-NAF1 antibody [EPR11516]
See all NAF1 primary antibodies
DescriptionRabbit monoclonal [EPR11516] to NAF1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC,IHC-P or IP
Species reactivityReacts with: Human
Does not react with: Mouse, Rat
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAF1 aa 450 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q96HR8
- HeLa, A431, A549, Namalwa and Caco-2 cell lysates; HeLa cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: BSA, Glycerol
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab171078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 54 kDa.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionRNA-binding protein required for the maturation of box H/ACA snoRNPs complex and ribosome biogenesis. During assembly of the H/ACA snoRNPs complex, it associates with the complex and disapears during maturation of the complex and is replaced by NOLA1/GAR1 to yield mature H/ACA snoRNPs complex. Probably competes with NOLA1/GAR1 for binding with DKC1/NOLA4.
Sequence similaritiesBelongs to the NAF1 family.
Cellular localizationCytoplasm. Nucleus. Shuttles between the cytoplasm and the nucleus. Absent from the nucleolus.
- Information by UniProt
- H/ACA ribonucleoprotein complex non-core subunit NAF1 antibody
- hNAF1 antibody
- Naf1 antibody
All lanes : Anti-NAF1 antibody [EPR11516] (ab171078) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A431 cell lysate
Lane 3 : A549 cell lysate
Lane 4 : Namalwa cell lysate
Lane 5 : Caco-2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 54 kDa
Flow cytometric analysis of permeabilized HeLa cells labeling NAF1 with ab171078 at 1/10 dilution (red), compared to a rabbit IgG negative control (green).
ab171078 has not yet been referenced specifically in any publications.