Overview

  • Product name

  • Description

    Rabbit polyclonal to NAGK
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Other Immunogen Type corresponding to Human NAGK aa 50-100.
    Database link: NP_060037.2

  • Positive control

    • Whole cell lysate from HeLa cells, 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab95182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the eukaryotic-type N-acetylglucosamine kinase family.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.1.59 antibody
    • GlcNAc kinase antibody
    • GNK antibody
    • HSA242910 antibody
    • N acetylglucosamine kinase antibody
    • N-acetyl-D-glucosamine kinase antibody
    • N-acetylglucosamine kinase antibody
    • nagK antibody
    • NAGK_HUMAN antibody
    see all

Images

  • All lanes : Anti-NAGK antibody (ab95182) at 0.4 µg/ml

    Lane 1 : Whole cell lysate from HeLa at 50 µg
    Lane 2 : Whole cell lysate from HeLa at 15 µg
    Lane 3 : Whole cell lysate from HeLa at 5 µg
    Lane 4 : 293T cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 37 kDa


    Exposure time: 30 seconds
  • Detection of Human NAGK by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) NAGK was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 30 seconds.

References

ab95182 has not yet been referenced specifically in any publications.

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