Key features and details
- Rabbit polyclonal to NAGK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NAGK antibody
See all NAGK primary antibodies
DescriptionRabbit polyclonal to NAGK
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Other Immunogen Type corresponding to Human NAGK aa 50-100.
Database link: NP_060037.2
- Whole cell lysate from HeLa cells, 293T cell lysate
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab95182 was affinity purified using an epitope specific to NAGK immobilized on solid support.
Our Abpromise guarantee covers the use of ab95182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 37 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionConverts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity.
Sequence similaritiesBelongs to the eukaryotic-type N-acetylglucosamine kinase family.
- Information by UniProt
- EC 18.104.22.168 antibody
- GlcNAc kinase antibody
- GNK antibody
All lanes : Anti-NAGK antibody (ab95182) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa at 50 µg
Lane 2 : Whole cell lysate from HeLa at 15 µg
Lane 3 : Whole cell lysate from HeLa at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds
Detection of Human NAGK by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) NAGK was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 30 seconds.
ab95182 has not yet been referenced specifically in any publications.