Anti-NAGK antibody (ab95182)
Key features and details
- Rabbit polyclonal to NAGK
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAGK antibody
See all NAGK primary antibodies -
Description
Rabbit polyclonal to NAGK -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Other Immunogen Type corresponding to Human NAGK aa 50-100.
Database link: NP_060037.2 -
Positive control
- Whole cell lysate from HeLa cells, 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab95182 was affinity purified using an epitope specific to NAGK immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
Target
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Function
Converts endogenous N-acetylglucosamine (GlcNAc), a major component of complex carbohydrates, from lysosomal degradation or nutritional sources into GlcNAc 6-phosphate. Also has ManNAc kinase activity. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the eukaryotic-type N-acetylglucosamine kinase family. - Information by UniProt
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Database links
- Entrez Gene: 55577 Human
- Omim: 606828 Human
- SwissProt: Q9UJ70 Human
- Unigene: 7036 Human
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Alternative names
- EC 2.7.1.59 antibody
- GlcNAc kinase antibody
- GNK antibody
see all
Images
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All lanes : Anti-NAGK antibody (ab95182) at 0.4 µg/ml
Lane 1 : Whole cell lysate from HeLa at 50 µg
Lane 2 : Whole cell lysate from HeLa at 15 µg
Lane 3 : Whole cell lysate from HeLa at 5 µg
Lane 4 : 293T cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds -
Detection of Human NAGK by Immunoprecipitation and Western Blot. Whole cell lysate from HeLa at 1 mg for IP, 20% of IP loaded. ab95182 was used for IP at 3 mcg/mg lysate and 1ug/ml for wb (lane 2) NAGK was also immunoprecipitated by another rabbit antibody (lane 1) and control IgG (lane 3). Detection: Chemiluminescence with exposure times of 30 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab95182 has not yet been referenced specifically in any publications.