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Rabbit polyclonal to NAGLU
Recombinant fragment, corresponding to a region within internal sequence amino acids 252-487 of Human NAGLU (UniProt: P54802).
Raji whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.01% Thimerosal (merthiolate) Constituents: 0.75% Glycine, 1.21% Tris, 20% Glycerol
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Involved in the degradation of heparan sulfate.
Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
Involvement in disease
Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
Information by UniProt
Alpha N acetylglucosaminidase antibody
alpha N acetylglucosaminidase, lysosomal antibody
Alpha-N-acetylglucosaminidase 77 kDa form antibody
Western blot - Anti-NAGLU antibody (ab154508)
Anti-NAGLU antibody (ab154508) at 1/1000 dilution + Raji whole cell
lysate at 30 µg Predicted band size: 82 kDa 7.5% SDS PAGE
Immunocytochemistry/ Immunofluorescence - Anti-NAGLU antibody (ab154508)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells (4% at RT, 15mins) labeling NAGLU with ab154508 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAGLU antibody (ab154508)
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling NAGLU with ab154508 at 1/500 dilution.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"