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  1. Link

    naglunag-antibody-ab137685.pdf

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Anti-NAGLU/NAG antibody (ab137685)

  • Datasheet
  • SDS
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Western blot - Anti-NAGLU/NAG antibody (ab137685)
  • Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody (ab137685)

Key features and details

  • Rabbit polyclonal to NAGLU/NAG
  • Suitable for: WB, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

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Overview

  • Product name

    Anti-NAGLU/NAG antibody
    See all NAGLU/NAG primary antibodies
  • Description

    Rabbit polyclonal to NAGLU/NAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Cow
  • Immunogen

    Recombinant fragment corresponding to Human NAGLU/NAG aa 101-558.
    Database link: P54802

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; paraformaldehyde-fixed HeLa cells.
  • General notes

     This product was previously labelled as NAGLU

     

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Sensory System
    • Visual system
    • Neuroscience
    • Sensory System
    • Auditory system
    • Signal Transduction
    • Cytoskeleton / ECM
    • HSPGs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • A431 whole cell lysate (ab7909)
    • MOLT4 whole cell lysate (ab7912)

Applications

Our Abpromise guarantee covers the use of ab137685 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Involved in the degradation of heparan sulfate.
  • Tissue specificity

    Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
  • Involvement in disease

    Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
  • Cellular localization

    Lysosome.
  • Target information above from: UniProt accession P54802 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 789125 Cow
    • Entrez Gene: 4669 Human
    • Entrez Gene: 27419 Mouse
    • Omim: 609701 Human
    • SwissProt: P54802 Human
    • Unigene: 50727 Human
    • Unigene: 95452 Mouse
    • Alternative names

      • Alpha N acetylglucosaminidase antibody
      • alpha N acetylglucosaminidase, lysosomal antibody
      • Alpha-N-acetylglucosaminidase 77 kDa form antibody
      • ANAG antibody
      • ANAG_HUMAN antibody
      • CMT2V antibody
      • MPS IIIB antibody
      • MPS3B antibody
      • N acetyl alpha glucosaminidase antibody
      • N acetylglucosaminidase, alpha antibody
      • N-acetyl-alpha-glucosaminidase antibody
      • NAG antibody
      • NAGLU antibody
      • UFHSD 1 antibody
      • UFHSD antibody
      • UFHSD1 antibody
      see all

    Images

    • Western blot - Anti-NAGLU/NAG antibody (ab137685)
      Western blot - Anti-NAGLU/NAG antibody (ab137685)
      All lanes : Anti-NAGLU/NAG antibody (ab137685) at 1/1000 dilution

      Lane 1 : H1299 whole cell lysate
      Lane 2 : HeLa S3 whole cell lysate
      Lane 3 : HepG2 whole cell lysate

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 82 kDa



      7.5% SDS PAGE
    • Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody (ab137685)
      Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody (ab137685)

      Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling NAGLU/NAG with ab137685 at 1/200 dilution.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab137685? Please let us know so that we can cite the reference in this datasheet.

    ab137685 has not yet been referenced specifically in any publications.

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