Anti-NAGLU/NAG antibody (ab137685)
Key features and details
- Rabbit polyclonal to NAGLU/NAG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAGLU/NAG antibody
See all NAGLU/NAG primary antibodies -
Description
Rabbit polyclonal to NAGLU/NAG -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Cow
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Immunogen
Recombinant fragment corresponding to Human NAGLU/NAG aa 101-558.
Database link: P54802 -
Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; paraformaldehyde-fixed HeLa cells.
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General notes
This product was previously labelled as NAGLU
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab137685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 82 kDa. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
Involved in the degradation of heparan sulfate. -
Tissue specificity
Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney. -
Involvement in disease
Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 789125 Cow
- Entrez Gene: 4669 Human
- Entrez Gene: 27419 Mouse
- Omim: 609701 Human
- SwissProt: P54802 Human
- Unigene: 50727 Human
- Unigene: 95452 Mouse
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Alternative names
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
see all
Images
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Western blot - Anti-NAGLU/NAG antibody (ab137685)All lanes : Anti-NAGLU/NAG antibody (ab137685) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-NAGLU/NAG antibody (ab137685)Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling NAGLU/NAG with ab137685 at 1/200 dilution.
Protocols
References (0)
ab137685 has not yet been referenced specifically in any publications.
