Key features and details
- Rabbit polyclonal to NAGLU/NAG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NAGLU/NAG antibody
See all NAGLU/NAG primary antibodies
DescriptionRabbit polyclonal to NAGLU/NAG
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
Recombinant fragment corresponding to Human NAGLU/NAG aa 101-558.
Database link: P54802
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4 and Raji whole cell lysates; paraformaldehyde-fixed HeLa cells.
This product was previously labelled as NAGLU
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab137685 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 82 kDa.|
|ICC/IF||1/100 - 1/1000.|
FunctionInvolved in the degradation of heparan sulfate.
Tissue specificityLiver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
Involvement in diseaseDefects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
- Information by UniProt
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
All lanes : Anti-NAGLU/NAG antibody (ab137685) at 1/1000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa S3 whole cell lysate
Lane 3 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 82 kDa
7.5% SDS PAGE
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells labelling NAGLU/NAG with ab137685 at 1/200 dilution.
ab137685 has not yet been referenced specifically in any publications.