Anti-NAGLU/NAG antibody (ab154508)
Key features and details
- Rabbit polyclonal to NAGLU/NAG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-NAGLU/NAG antibody
See all NAGLU/NAG primary antibodies -
Description
Rabbit polyclonal to NAGLU/NAG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NAGLU/NAG aa 252-487 (internal sequence).
Database link: P54802 -
Positive control
- Raji whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 82 kDa.
|
|
IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
|
ICC/IF |
1/500.
|
Notes |
---|
WB
1/500 - 1/3000. Predicted molecular weight: 82 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/500. |
Target
-
Function
Involved in the degradation of heparan sulfate. -
Tissue specificity
Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney. -
Involvement in disease
Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. -
Cellular localization
Lysosome. - Information by UniProt
-
Database links
- Entrez Gene: 4669 Human
- Omim: 609701 Human
- SwissProt: P54802 Human
- Unigene: 50727 Human
-
Alternative names
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
see all
Images
-
Anti-NAGLU/NAG antibody (ab154508) at 1/1000 dilution + Raji whole cell
lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE -
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells (4% at RT, 15mins) labeling NAGLU/NAG with ab154508 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
-
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling NAGLU/NAG with ab154508 at 1/500 dilution.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab154508 has not yet been referenced specifically in any publications.