Overview

  • Product name

  • Description

    Rabbit polyclonal to NAGLU/NAG
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NAGLU/NAG aa 252-487 (internal sequence).
    Database link: P54802

  • Positive control

    • Raji whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue
  • General notes

     This product was previously labelled as NAGLU

     

Properties

Applications

Our Abpromise guarantee covers the use of ab154508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 82 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/500.

Target

  • Function

    Involved in the degradation of heparan sulfate.
  • Tissue specificity

    Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
  • Involvement in disease

    Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
  • Cellular localization

    Lysosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • Alpha N acetylglucosaminidase antibody
    • alpha N acetylglucosaminidase, lysosomal antibody
    • Alpha-N-acetylglucosaminidase 77 kDa form antibody
    • ANAG antibody
    • ANAG_HUMAN antibody
    • CMT2V antibody
    • MPS IIIB antibody
    • MPS3B antibody
    • N acetyl alpha glucosaminidase antibody
    • N acetylglucosaminidase, alpha antibody
    • N-acetyl-alpha-glucosaminidase antibody
    • NAG antibody
    • NAGLU antibody
    • UFHSD 1 antibody
    • UFHSD antibody
    • UFHSD1 antibody
    see all

Images

  • Anti-NAGLU/NAG antibody (ab154508) at 1/1000 dilution + Raji whole cell
    lysate at 30 µg

    Predicted band size: 82 kDa



    7.5% SDS PAGE
  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells (4% at RT, 15mins) labeling NAGLU/NAG with ab154508 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.

  • Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling NAGLU/NAG with ab154508 at 1/500 dilution.

References

ab154508 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab154508.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up