Key features and details
- Rabbit polyclonal to NAGLU/NAG
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NAGLU/NAG antibody
See all NAGLU/NAG primary antibodies
DescriptionRabbit polyclonal to NAGLU/NAG
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NAGLU/NAG aa 252-487 (internal sequence).
Database link: P54802
- Raji whole cell lysate; HeLa cells; Human lung adenocarcinoma tissue
This product was previously labelled as NAGLU
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154508 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 82 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionInvolved in the degradation of heparan sulfate.
Tissue specificityLiver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
Involvement in diseaseDefects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
- Information by UniProt
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
Anti-NAGLU/NAG antibody (ab154508) at 1/1000 dilution + Raji whole cell
lysate at 30 µg
Predicted band size: 82 kDa
7.5% SDS PAGE
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells (4% at RT, 15mins) labeling NAGLU/NAG with ab154508 at 1/500 dilution. Right image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human lung adenocarcinoma tissue labeling NAGLU/NAG with ab154508 at 1/500 dilution.
ab154508 has not yet been referenced specifically in any publications.