Recombinant Anti-NAGLU/NAG antibody [EPR20708] (ab214671)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20708] to NAGLU/NAG
- Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-NAGLU/NAG antibody [EPR20708]
See all NAGLU/NAG primary antibodies -
Description
Rabbit monoclonal [EPR20708] to NAGLU/NAG -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
This product was produced with the following immunogens:
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
Positive control
- WB: Human placenta, liver and fetal kidney lysates; HepG2 whole cell lysate; Mouse colon and testis lysates; Rat colon, testis, liver and spleen lysates. IHC-P: Human kidney and liver tissues; Mouse and rat kidney tissues. ICC/IF: HepG2 and Hepa1-6 cells. Flow Cyt (intra): Hepa1-6 and HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.05% BSA, 40% Glycerol, PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20708 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab214671 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/50.
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WB |
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).
Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed. |
|
ICC/IF |
1/100.
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IHC-P |
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/50. |
WB
1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa). Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed. |
ICC/IF
1/100. |
IHC-P
1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the degradation of heparan sulfate. -
Tissue specificity
Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney. -
Involvement in disease
Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life. -
Cellular localization
Lysosome. - Information by UniProt
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Database links
- Entrez Gene: 4669 Human
- Entrez Gene: 27419 Mouse
- Entrez Gene: 360630 Rat
- Omim: 609701 Human
- SwissProt: P54802 Human
- Unigene: 50727 Human
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Alternative names
- Alpha N acetylglucosaminidase antibody
- alpha N acetylglucosaminidase, lysosomal antibody
- Alpha-N-acetylglucosaminidase 77 kDa form antibody
see all
Images
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Lanes 1-3 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution
Lane 4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/5000 dilution
Lane 1 : Human placenta lysate at 20 µg
Lane 2 : Human liver lysate at 20 µg
Lane 3 : Human fetal kidney lysate at 10 µg
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg
Secondary
Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 82 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.
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Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Lanes 1-4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/2000 dilution
Lanes 5-6 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution
Lane 1 : Mouse colon lysate at 20 µg
Lane 2 : Rat testis lysate at 20 µg
Lane 3 : Mouse testis lysate at 20 µg
Lane 4 : Rat colon lysate at 20 µg
Lane 5 : Rat liver lysate at 10 µg
Lane 6 : Rat spleen lysate at 10 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Developed using the ECL technique.
Predicted band size: 82 kDa
Observed band size: 82 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: Lanes 1-2,5-6: 3 minutes; Lanes 3-4: 30 seconds.
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Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human liver (PMID: 4291567, PMID: 8776591). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on rat kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 100% methanol-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
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Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab214671 has been referenced in 2 publications.
- Xing C et al. Downregulation of NAGLU in VEC Increases Abnormal Accumulation of Lysosomes and Represents a Predictive Biomarker in Early Atherosclerosis. Front Cell Dev Biol 9:797047 (2021). PubMed: 35155448
- De Pasquale V et al. EGFR activation triggers cellular hypertrophy and lysosomal disease in NAGLU-depleted cardiomyoblasts, mimicking the hallmarks of mucopolysaccharidosis IIIB. Cell Death Dis 9:40 (2018). PubMed: 29348482