Overview

  • Product name
    Anti-NAGLU/NAG antibody [EPR20708]
    See all NAGLU/NAG primary antibodies
  • Description
    Rabbit monoclonal [EPR20708] to NAGLU/NAG
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide within Human NAGLU/NAG aa 100-200. The exact sequence is proprietary.
    Database link: P54802

    Synthetic peptide within Human NAGLU/NAG aa 1-100. The exact sequence is proprietary.
    Database link: P54802

  • Positive control
    • WB: Human placenta, liver and fetal kidney lysates; HepG2 whole cell lysate; Mouse colon and testis lysates; Rat colon, testis, liver and spleen lysates. IHC-P: Human kidney and liver tissues; Mouse and rat kidney tissues. ICC/IF: HepG2 and Hepa1-6 cells. Flow Cyt: Hepa1-6 and HepG2 cells.
  • General notes

     This product was previously labelled as NAGLU

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab214671 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 82 kDa (predicted molecular weight: 82 kDa).

Additional bands observed in some rodent tissue lysates where NAGLU is not highly expressed.

ICC/IF 1/100.
Flow Cyt 1/50.
IHC-P 1/4000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function
    Involved in the degradation of heparan sulfate.
  • Tissue specificity
    Liver, ovary, peripheral blood leukocytes, testis, prostate, spleen, colon, lung, placenta and kidney.
  • Involvement in disease
    Defects in NAGLU are the cause of mucopolysaccharidosis type 3B (MPS3B) [MIM:252920]; also known as Sanfilippo syndrome B. MPS3B is a form of mucopolysaccharidosis type 3, an autosomal recessive lysosomal storage disease due to impaired degradation of heparan sulfate. MPS3 is characterized by severe central nervous system degeneration, but only mild somatic disease. Onset of clinical features usually occurs between 2 and 6 years; severe neurologic degeneration occurs in most patients between 6 and 10 years of age, and death occurs typically during the second or third decade of life.
  • Cellular localization
    Lysosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha N acetylglucosaminidase antibody
    • alpha N acetylglucosaminidase, lysosomal antibody
    • Alpha-N-acetylglucosaminidase 77 kDa form antibody
    • ANAG antibody
    • ANAG_HUMAN antibody
    • CMT2V antibody
    • MPS IIIB antibody
    • MPS3B antibody
    • N acetyl alpha glucosaminidase antibody
    • N acetylglucosaminidase, alpha antibody
    • N-acetyl-alpha-glucosaminidase antibody
    • NAG antibody
    • NAGLU antibody
    • UFHSD 1 antibody
    • UFHSD antibody
    • UFHSD1 antibody
    see all

Images

  • Lanes 1-3 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution
    Lane 4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/5000 dilution

    Lane 1 : Human placenta lysate at 20 µg
    Lane 2 : Human liver lysate at 20 µg
    Lane 3 : Human fetal kidney lysate at 10 µg
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate at 10 µg

    Secondary
    Lanes 1-2 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution
    Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2: 1 minute; Lanes 3-4: 30 seconds.

  • Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunofluorescent analysis of 100% methanol-fixed HepG2 (human liver hepatocellular carcinoma cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Lanes 1-4 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/2000 dilution
    Lanes 5-6 : Anti-NAGLU/NAG antibody [EPR20708] (ab214671) at 1/1000 dilution

    Lane 1 : Mouse colon lysate at 20 µg
    Lane 2 : Rat testis lysate at 20 µg
    Lane 3 : Mouse testis lysate at 20 µg
    Lane 4 : Rat colon lysate at 20 µg
    Lane 5 : Rat liver lysate at 10 µg
    Lane 6 : Rat spleen lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Developed using the ECL technique.

    Predicted band size: 82 kDa
    Observed band size: 82 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lanes 1-2,5-6: 3 minutes; Lanes 3-4: 30 seconds.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on human liver (PMID: 4291567, PMID: 8776591). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on mouse kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling NAGLU/NAG with ab214671 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Granular cytoplasmic staining on rat kidney tubules (PMID: 4291567, PMID: 24244710). Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

  • Immunofluorescent analysis of 100% methanol-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cells labeling NAGLU/NAG with ab214671 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on Hepa1-6 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed Hepa1-6 (mouse hepatoma epithelial cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

  • Flow cytometric analysis of 4% paraformaldehyde-fixed HepG2 (human liver hepatocellular carcinoma cell line) cell line labeling NAGLU/NAG with ab214671at 1/50 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/2000 dilution was used as the secondary antibody.

References

This product has been referenced in:
  • De Pasquale V  et al. EGFR activation triggers cellular hypertrophy and lysosomal disease in NAGLU-depleted cardiomyoblasts, mimicking the hallmarks of mucopolysaccharidosis IIIB. Cell Death Dis 9:40 (2018). Read more (PubMed: 29348482) »
See 1 Publication for this product

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