Key features and details
- Rabbit polyclonal to NAGPA
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NAGPA antibody
DescriptionRabbit polyclonal to NAGPA
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human NAGPA aa 327-438.
Database link: Q9UK23
- WB: SH-SY5Y, PC-3, A549 and HepG2 whole cell lysates. IHC-P: Human colon cancer tissue.
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab234978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000.|
|WB||1/500 - 1/5000. Predicted molecular weight: 56 kDa.|
FunctionCatalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step.
Tissue specificityIsoform 2 may be brain-specific.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesContains 1 EGF-like domain.
DomainThe tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.
The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.
Cellular localizationGolgi apparatus > Golgi stack membrane. Cis/medial Golgi.
- Information by UniProt
- Mannose 6-phosphate-uncovering enzyme antibody
- N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase antibody
- NAGPA antibody
All lanes : Anti-NAGPA antibody (ab234978) at 1/500 dilution
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 56 kDa
Paraffin-embedded human colon cancer tissue stained for NAGPA with ab234978 at 1/500 dilution in immunohistochemical analysis.
After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab234978 has not yet been referenced specifically in any publications.