Overview

  • Product name

    Anti-NAGPA antibody
  • Description

    Rabbit polyclonal to NAGPA
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human NAGPA aa 327-438.
    Database link: Q9UK23

  • Positive control

    • WB: SH-SY5Y, PC-3, A549 and HepG2 whole cell lysates. IHC-P: Human colon cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab234978 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000.
WB 1/500 - 1/5000. Predicted molecular weight: 56 kDa.

Target

  • Function

    Catalyzes the second step in the formation of the mannose 6-phosphate targeting signal on lysosomal enzyme oligosaccharides by removing GlcNAc residues from GlcNAc-alpha-P-mannose moieties, which are formed in the first step.
  • Tissue specificity

    Isoform 2 may be brain-specific.
  • Pathway

    Protein modification; protein glycosylation.
  • Sequence similarities

    Contains 1 EGF-like domain.
  • Domain

    The tyrosine-based internalization signal may be essential for its retrieval from the plasma membrane to the TGN.
    The C-terminal NPFKD sequence is an attractive candidate for either an endocytosis signal acting at the plasma membrane or a retrieval signal acting at the TGN to return the enzyme to the cis/medial-Golgi.
  • Cellular localization

    Golgi apparatus > Golgi stack membrane. Cis/medial Golgi.
  • Information by UniProt
  • Database links

  • Alternative names

    • Mannose 6-phosphate-uncovering enzyme antibody
    • N-acetylglucosamine-1-phosphodiester alpha-N-acetylglucosaminidase antibody
    • NAGPA antibody
    • NAGPA_HUMAN antibody
    • Phosphodiester alpha-GlcNAcase antibody
    see all

Images

  • All lanes : Anti-NAGPA antibody (ab234978) at 1/500 dilution

    Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
    Lane 2 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 3 : A549 (Human lung carcinoma cell line) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution

    Predicted band size: 56 kDa

  • Paraffin-embedded human colon cancer tissue stained for NAGPA with ab234978 at 1/500 dilution in immunohistochemical analysis.

    After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

References

ab234978 has not yet been referenced specifically in any publications.

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