Key features and details
- Rabbit polyclonal to NAIP
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-NAIP antibody
See all NAIP primary antibodies
DescriptionRabbit polyclonal to NAIP
SpecificityThis antibody detects recombinant human NAIP in extracts from Sf21 insect cells, but it is unknown as to whether this antibody detects the endogenous form.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Synthetic peptide surrounding amino acid 506 of human NAIP.
NAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Primary antibody notesNAIP (Neuronal Apoptosis Inhibitor Protein) is a member of the IAPs (Inhibitor of Apoptosis Proteins) that function in cell death pathways to inhibit apoptosis. NAIP was discovered based on its association with a neurodegenerative disorder. Mutations and deletions in the NAIP gene locus is a contributing factor in spinal muscular atrophy. Coexpression of NAIP and hippocalcin protects neurons against calcium-induced cell death. NAIP is strongly expressed in anterior horn and motor cortex neurons of the normal brain. It is also found in human fetal neurons and in adult choroid plexus cells.
Our Abpromise guarantee covers the use of ab2549 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 140 kDa.|
FunctionPrevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Tissue specificityExpressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
Sequence similaritiesContains 3 BIR repeats.
Contains 1 NACHT domain.
- Information by UniProt
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
All lanes : Anti-NAIP antibody (ab2549) at 1/1000 dilution
Lane 1 : Lysate prepared from transfected PC3 cells.
Lane 2 : Lysate prepared from non-transfected PC3 cells.
Lane 3 : Lysate prepared from transfected HeLa cells.
Lane 4 : Lysate prepared from non-transfected HeLa cells.
Lane 5 : Lysate prepared from non-transfected NTera2D1 cells.
All lanes : secondary antibody at 1/100000 dilution
Observed band size: 160 kDa why is the actual band size different from the predicted?
Additional bands at: 105 kDa (possible isoform), 115 kDa, 130 kDa (possible isoform). We are unsure as to the identity of these extra bands.
Detection of novel NAIP protein isoforms.Cells were grown in 10 cm dishes. The human PC3, NTera2D1, and HeLa cell lines were selected to screen for NAIP proteins based on preliminary RT-PCR findings. Cells transfected with the expression vector encoding the Alu-derived NAIP ORF or untransfected controls were harvested by either scraping or trypsinization following two washes with cold PBS. Cell pellets were obtained by centrifugation and resuspended in RIPA (150 mM NaCl; 1% NP-40; 0.5% sodium deoxycholate; 0.1% SDS; 50 mM Tris, pH8) and NP40 (150 mM NaCl; 1% NP-40; 50 mM Tris, pH8) lysis buffers supplemented with a protease inhibitor cocktail, and subsequently quantified using the Qubit Fluorometer. RIPA provided clearer results for the NAIP-specific antibody. Bi-phased gels containing TEMED and APS (4% stacking, 9% separating) were used to resolve total cellular protein in electrophoresis running buffer (10×: 25 mM Tris; 192 mM glycine; 0.1% SDS). Subsequently, separa
ab2549 has been referenced in 1 publication.
- Romanish MT et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB ; Human . PubMed: 19488400