Product nameAnti-NAIP antibody
See all NAIP primary antibodies
DescriptionRabbit polyclonal to NAIP
Specificityab25968 recognises Neuronal apoptosis inhibitor protein (NAIP).
Tested applicationsSuitable for: WB, IHC-P, ELISA, ICC/IFmore details
Species reactivityReacts with: Human
Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).
- PC-3 cell lysate
Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab25968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).Can be blocked with Human NAIP peptide (ab39795).|
|IHC-P||Use at an assay dependent concentration. PubMed: 20844241|
|ELISA||Use at an assay dependent concentration.|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionPrevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy.
Tissue specificityExpressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord.
Sequence similaritiesContains 3 BIR repeats.
Contains 1 NACHT domain.
- Information by UniProt
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1µg/ml.
Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms.
ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF
IHC image of ab25968 staining in human normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab25968, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa (possible non-specific binding)
Exposure time: 1 minute
Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.
This product has been referenced in:
- Abadía-Molina F et al. Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division. Sci Rep 7:39981 (2017). Read more (PubMed: 28059125) »
- Engel K et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med 8:851-62 (2016). Read more (PubMed: 27317434) »