Anti-NAIP antibody (ab25968)
Key features and details
- Rabbit polyclonal to NAIP
- Suitable for: WB, ELISA, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAIP antibody
See all NAIP primary antibodies -
Description
Rabbit polyclonal to NAIP -
Host species
Rabbit -
Specificity
ab25968 recognises Neuronal apoptosis inhibitor protein (NAIP). -
Tested applications
Suitable for: WB, ELISA, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to 13 C terminal amino acids of Human NAIP (Genbank accession No. AAC52047).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa).
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ELISA |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. PubMed: 20844241
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 160 kDa (predicted molecular weight: 160 kDa). |
ELISA
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. PubMed: 20844241 |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Prevents motor-neuron apoptosis induced by a variety of signals. Possible role in the prevention of spinal muscular atrophy that seems to be caused by inappropriate persistence of motor-neuron apoptosis: mutated or deleted forms of NAIP have been found in individuals with severe spinal muscular atrophy. -
Tissue specificity
Expressed in motor neurons, but not in sensory neurons. Found in liver and placenta, and to a lesser extent in spinal cord. -
Sequence similarities
Contains 3 BIR repeats.
Contains 1 NACHT domain. - Information by UniProt
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Database links
- Entrez Gene: 100510692 Human
- Entrez Gene: 4671 Human
- Omim: 600355 Human
- SwissProt: Q13075 Human
- Unigene: 654500 Human
- Unigene: 710305 Human
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Alternative names
- Baculoviral IAP repeat containing 1 antibody
- Baculoviral IAP repeat-containing protein 1 antibody
- BIRC 1 antibody
see all
Images
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Western Blot of human tonsil lysate labeling NAIP with Anti-NAIP antibody (ab25968) at 1µg/ml.
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Western blot analysis of NAIP in PC-3 cell lysate with anti-NAIP (CT) at (A) 0.5, (B) 1, and (C) 2 µg/ml.
Minor, lower molecular weight bands may represent alternately spliced forms. -
ab25968 at 10µg/ml staining NAIP in A549 cells by ICC/IF
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Anti-NAIP antibody (ab25968) at 1/1500 dilution + Mouse RAW 264.7 cell lysate at 20 µg
Secondary
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 160 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Additional bands at: 110 kDa (possible non-specific binding)
Exposure time: 1 minute
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Immunofluorescence of NAIP in A549 cells using ab25968 at 20 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab25968 has been referenced in 10 publications.
- Zhao Z et al. Early Pregnancy Modulates Expression of the Nod-like Receptor Family in Lymph Nodes of Ewes. Animals (Basel) 12:N/A (2022). PubMed: 36496806
- Triantafilou K et al. Differential recognition of HIV-stimulated IL-1ß and IL-18 secretion through NLR and NAIP signalling in monocyte-derived macrophages. PLoS Pathog 17:e1009417 (2021). PubMed: 33861800
- Huang Z et al. Inhibition of caspase-3-mediated GSDME-derived pyroptosis aids in noncancerous tissue protection of squamous cell carcinoma patients during cisplatin-based chemotherapy. Am J Cancer Res 10:4287-4307 (2020). PubMed: 33415000
- Abadía-Molina F et al. Neuronal apoptosis inhibitory protein (NAIP) localizes to the cytokinetic machinery during cell division. Sci Rep 7:39981 (2017). PubMed: 28059125
- Engel K et al. USP9X stabilizes XIAP to regulate mitotic cell death and chemoresistance in aggressive B-cell lymphoma. EMBO Mol Med 8:851-62 (2016). PubMed: 27317434
- Zhang S et al. Apollon modulates chemosensitivity in human esophageal squamous cell carcinoma. Oncotarget 5:7183-97 (2014). PubMed: 25216531
- Petterson T et al. Effects of NOD-like receptors in human B lymphocytes and crosstalk between NOD1/NOD2 and Toll-like receptors. J Leukoc Biol 89:177-87 (2011). IHC-P ; Human . PubMed: 20844241
- Chiu HH et al. Induction of neuronal apoptosis inhibitory protein expression in response to androgen deprivation in prostate cancer. Cancer Lett 292:176-85 (2010). WB ; Mouse . PubMed: 20044205
- Romanish MT et al. A novel protein isoform of the multicopy human NAIP gene derives from intragenic Alu SINE promoters. PLoS One 4:e5761 (2009). WB ; Human . PubMed: 19488400
- Hebb AL et al. Expression of the inhibitor of apoptosis protein family in multiple sclerosis reveals a potential immunomodulatory role during autoimmune mediated demyelination. Mult Scler 14:577-94 (2008). PubMed: 18566024