Overview

  • Product name

    Anti-Naked1 antibody [EPR8603]
    See all Naked1 primary antibodies
  • Description

    Rabbit monoclonal [EPR8603] to Naked1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to a region within Human Naked1 (UniProt: Q969G9).

  • Positive control

    • HepG2, SW480 and 293T cell lysates; Human fetal kidney tissue lysate
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR8603
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133650 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/10000 - 1/50000. Predicted molecular weight: 52 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Relevance

      Naked1 (Nkd1) is a homologue of Drosophila Naked cuticle, a negative regulator of Wnt/Wingless signaling pathway. Naked proteins contain a putative calcium-binding EF-hand motif; however, Drosophila Naked binds to zinc instead of calcium. Naked inhibits the canonical Wnt/ß-catenin pathway by binding to Dishevelled proteins and directs Dishevelled activity towards the planar cell polarity pathway. Naked1 is a direct target of Wnt signaling and is overexpressed in some colon tumors due to constitutive activation of Wnt/ß-catenin pathway.
    • Cellular localization

      Cell membrane
    • Database links

    • Alternative names

      • Dvl-binding protein antibody
      • Naked cuticle 1 antibody
      • naked cuticle 1 homolog antibody
      • Naked cuticle homolog 1 (Drosophila) antibody
      • Naked-1 antibody
      • NKD antibody
      • Nkd1 antibody
      • Protein naked cuticle homolog 1 antibody
      see all

    Images

    • Immunocytochemistry/Immunofluorescence analysis of HepG2 (Human liver hepatocellular carcinoma cell line) labeling Naked1 with Purified ab133650 at 1/500 dilution (5 µg/ml). Cells were fixed with 4% PFA and permeabilized with 0.1% tritonX-100. ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) at 1/1000 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

    • All lanes : Anti-Naked1 antibody [EPR8603] (ab133650) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : SW480 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : Human fetal kidney tissue lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 52 kDa

    References

    This product has been referenced in:

    • Lu Y  et al. miR-205 Regulates Basal Cell Identity and Stem Cell Regenerative Potential During Mammary Reconstitution. Stem Cells 36:1875-1889 (2018). Read more (PubMed: 30267595) »
    • Ma S  et al. SKLB-677, an FLT3 and Wnt/ß-catenin signaling inhibitor, displays potent activity in models of FLT3-driven AML. Sci Rep 5:15646 (2015). WB . Read more (PubMed: 26497577) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Oviduct)
    Permeabilization
    Yes - 0.1% Triton
    Specification
    Oviduct
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde

    Abcam user community

    Verified customer

    Submitted May 03 2018

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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