Anti-NAK/TBK1 antibody [108A429] (ab12116)
![Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)](https://www.abcam.com/ps/products/12/ab12116/Images/ab12116-4-anti-tbk1-antibody-108a429-western-blot-wildtype-u2os-knockout-tbk1-human.jpg "Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)")
Key features and details
- Mouse monoclonal [108A429] to NAK/TBK1
- Suitable for: Flow Cyt, WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-NAK/TBK1 antibody [108A429]
See all NAK/TBK1 primary antibodies -
Description
Mouse monoclonal [108A429] to NAK/TBK1 -
Host species
Mouse -
Specificity
ab12116 recognises human NAK. -
Tested applications
Suitable for: Flow Cyt, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
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Positive control
- 293 cell extracts transfected with human TBK1 cDNA.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
Affinity purified. -
Clonality
Monoclonal -
Clone number
108A429 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab12116 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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| WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa).
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| Notes |
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Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 80 kDa (predicted molecular weight: 87.5 kDa). |
Target
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Function
Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively. -
Tissue specificity
Ubiquitous with higher expression in testis. -
Sequence similarities
Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 29110 Human
- Omim: 604834 Human
- SwissProt: Q9UHD2 Human
- Unigene: 505874 Human
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Alternative names
- EC 2.7.11.1 antibody
- FLJ11330 antibody
- FTDALS4 antibody
see all
Images
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Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)
ab12116 was shown to react with TBK1 in wild-type U2OSn cells in Western blot with loss of signal observed in a TBK1 knockout cell line. Wild-type U2OSn and TBK1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 5% milk in TBST for 1 hr before incubation with ab12116 overnight at 4 °C at a 1/600 dilution. Blots were incubated with goat anti-mouse HRP secondary antibodies at 1/5000 before imaging. These data were provided by YCharOS Inc., an open science company with the mission of characterizing commercially available antibody reagents for all human proteins. Abcam and YCharOS are working together to help address the reproducibility crisis by enabling the life science community to better evaluate commercially available antibodies.
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![Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)](https://www.abcam.com/ps/products/12/ab12116/Images/ab12116-261806-anti-nak-tbk1-antibody-108a429-western-blot.jpg)
Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: NAK knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HepG2 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab12116 observed at 90 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab12116 was shown to recognize NAK when NAK knockout samples were used, along with additional cross-reactive bands. Wild-type and NAK knockout samples were subjected to SDS-PAGE. ab12116 and ab181602 (loading control to GAPDH) were diluted at 1 μg/ml and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging. -
![Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)](https://www.abcam.com/ps/products/12/ab12116/Images/ab12116-19494-anti-nak-tbk1-antibody-108a429-western-blot.jpg)
Western blot - Anti-NAK/TBK1 antibody [108A429] (ab12116)All lanes : Anti-NAK/TBK1 antibody [108A429] (ab12116) at 2 µg/ml
Lane 1 : 293 cells
Lane 2 : 293 cells transfected with Human NAK cDNA
Predicted band size: 87.5 kDa -
![Flow Cytometry - Anti-NAK/TBK1 antibody [108A429] (ab12116)](https://www.abcam.com/ps/products/12/ab12116/Images/ab12116-19493-anti-nak-tbk1-antibody-108a429-flow-cytometry.jpg)
Flow Cytometry - Anti-NAK/TBK1 antibody [108A429] (ab12116)Overlay histogram showing HeLa cells stained with ab12116 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab12116, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab12116 has been referenced in 6 publications.
- Hu L et al. TBK1 Is a Synthetic Lethal Target in Cancer with VHL Loss. Cancer Discov 10:460-475 (2020). PubMed: 31810986
- Ahn J et al. STING-Dependent Signaling Underlies IL-10 Controlled Inflammatory Colitis. Cell Rep 21:3873-3884 (2017). PubMed: 29281834
- Lio CW et al. cGAS-STING Signaling Regulates Initial Innate Control of Cytomegalovirus Infection. J Virol 90:7789-97 (2016). PubMed: 27334590
- Charoenthongtrakul S et al. RING finger protein 11 targets TBK1/IKKi kinases to inhibit antiviral signaling. PLoS One 8:e53717 (2013). WB . PubMed: 23308279
- Gao L et al. ABIN1 Protein Cooperates with TAX1BP1 and A20 Proteins to Inhibit Antiviral Signaling. J Biol Chem 286:36592-602 (2011). WB, IP . PubMed: 21885437
- Parvatiyar K et al. TAX1BP1 and A20 inhibit antiviral signaling by targeting TBK1-IKKi kinases. J Biol Chem 285:14999-5009 (2010). WB, IP ; Human . PubMed: 20304918