Key features and details
- Rabbit Polyclonal to NAK/TBK1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NAK/TBK1 antibody
See all NAK/TBK1 primary antibodies
DescriptionRabbit Polyclonal to NAK/TBK1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human NAK/TBK1 aa 364-585.
GRLAQHFPKTTEENPIFVVSREPLNTIGLIYEKISLPKVHPRYDLDGDAS MAKAITGVVCYACRIASTLLLYQELMRKGIRWLIELIKDDYNETVHKKTE VVITLDFCIRNIEKTVKVYEKLMKINLEAAELGEISDIHTKLLRLSSSQG TIETSLQDIDSRLSPGGSLADAWAHQEGTHPKDRNVEKLQVLLNCMTEIY YQFKKDKAERRLAYNEEQIHKF
Database link: Q9UHD2
- WB: Mouse liver and brain lysates. IHC-P: Human testis tissue. ICC/IF: HeLa (human epithelial cell line from cervix adenocarcinoma) cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab222809 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|ICC/IF||1/50 - 1/200.|
|WB||1/2000 - 1/5000. Detects a band of approximately 84 kDa (predicted molecular weight: 84 kDa).|
FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
Tissue specificityUbiquitous with higher expression in testis.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- EC 184.108.40.206 antibody
- FLJ11330 antibody
- FTDALS4 antibody
All lanes : Anti-NAK/TBK1 antibody (ab222809) at 1/2000 dilution
Lane 1 : Mouse liver lysate
Lane 2 : Mouse brain lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 84 kDa
Paraffin-embedded human testis tissue stained for NAK/TBK1 using ab222809 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NAK/TBK1 (green) using ab222809 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
ab222809 has not yet been referenced specifically in any publications.