Overview

  • Product name

  • Description

    Rabbit polyclonal to NAK/TBK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Pig, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human NAK/TBK1 (internal sequence). The exact sequence is proprietary.
    Database link: Q9UHD2

  • Positive control

    • WB: NT2D1, HeLa and HepG2 whole cell lysates; mouse testis tissue extract. IHC-P: Human H441 xenograft tissue; mouse testis tissue. ICC/IF: HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab227182 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 84 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
  • Tissue specificity

    Ubiquitous with higher expression in testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.11.1 antibody
    • FLJ11330 antibody
    • FTDALS4 antibody
    • NAK antibody
    • NF kappa B activating kinase antibody
    • NF kB activating kinase antibody
    • NF-kappa-B-activating kinase antibody
    • Serine/threonine protein kinase TBK 1 antibody
    • Serine/threonine protein kinase TBK1 antibody
    • Serine/threonine-protein kinase TBK1 antibody
    • T2K antibody
    • TANK binding kinase 1 antibody
    • TANK-binding kinase 1 antibody
    • TBK 1 antibody
    • Tbk1 antibody
    • TBK1_HUMAN antibody
    see all

Images

  • Anti-NAK/TBK1 antibody (ab227182) at 1/1000 dilution + NT2D1 (human malignant pluripotent embryonic carcinoma cell line) whole cell extract at 30 µg

    Predicted band size: 84 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-NAK/TBK1 antibody (ab227182) at 1/1000 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 84 kDa



    7.5% SDS-PAGE gel.

  • Anti-NAK/TBK1 antibody (ab227182) at 1/500 dilution + Mouse testis tissue extract at 50 µg

    Predicted band size: 84 kDa



    7.5% SDS-PAGE gel.

  • Paraffin-embedded mouse testis tissue stained for NAK/TBK1 using ab227182 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human H441 xenograft tissue stained for NAK/TBK1 using ab227182 at 1/500 dilution in immunohistochemical analysis.

  • 4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NAK/TBK1 (green) using ab227182 at 1/100 dilution in ICC/IF.

    Nuclear Counterstain: Hoechst 33342.

References

ab227182 has not yet been referenced specifically in any publications.

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