Key features and details
- Rabbit polyclonal to NAK/TBK1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-NAK/TBK1 antibody
See all NAK/TBK1 primary antibodies
DescriptionRabbit polyclonal to NAK/TBK1
Tested applicationsSuitable for: ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide. This information is considered to be commercially sensitive.
- WB: NIH/3T3 cell lysate. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab235253 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.|
|WB||1/1000. Predicted molecular weight: 84 kDa.|
FunctionSerine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
Tissue specificityUbiquitous with higher expression in testis.
Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
Contains 1 protein kinase domain.
- Information by UniProt
- EC 184.108.40.206 antibody
- FLJ11330 antibody
- FTDALS4 antibody
4% formaldehyde-fixed, 0.3% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for NAK/TBK1 (green) using ab235253 at 15 µg/ml overnight at 4⁰C, in ICC/IF, followed by Donkey Anti-Rabbit IgG DyLight™ 488 Conjugated Preadsorbed at 5 ug/ml 1 hour at room temperature. Counter-stained with DAPI.
(B) ab235253 + sec Dylight488
(C) Merge A+B
(D) Secondary only
Expected localization: cytoplasm.
Anti-NAK/TBK1 antibody (ab235253) at 1/1000 dilution + NIH/3T3 (mouse embyro fibroblast cell line) cell lysate
Rabbit IgG HRP at 1/70000 dilution
Predicted band size: 84 kDa
Exposure time: 30 seconds
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235253 has not yet been referenced specifically in any publications.