Recombinant
RabMAb

Recombinant Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free (ab186469)

Overview

  • Product name

    Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] - BSA and Azide free
    See all NAK/TBK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR2867(2)] to NAK/TBK1 (phospho S172) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    This antibody only detects NAK/TBK1 phosphorylated at serine 172.
  • Tested applications

    Suitable for: Dot blot, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NAK/TBK1 aa 150-250 (phospho S172). The exact sequence is proprietary.
    Database link: Q9UHD2
    (Peptide available as ab206124)

  • Positive control

    • WB: NIH/3T3 treated with 10mM pervanadate for 30 minutes, whole cell lysate.
  • General notes

    Ab186469 is the carrier-free version of ab109272. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab186469 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 84 kDa.Can be blocked with NAK/TBK1 (phospho S172) peptide (ab206124).
IP Use at an assay dependent concentration.

Target

  • Function

    Serine/threonine protein involved in the signaling cascade converging to the activation of the transcription factor NF-kappa-B. May function as an IKK kinase, playing an essential role in the transcription of a subset of TNF-alpha-induced genes. Also mediates production of RANTES/CCL5 and interferon-beta/IFNB1. Has a pivotal role in the innate immune response. Phosphorylates Borna disease virus (BDV) P protein. Phosphorylates and activates IRF3 and IRF7 and allows their nuclear localization. This leads to production of alpha/beta interferons and the development of a cellular antiviral state. It also seems to be a central factor in the induction of the antiviral interferon response. Inhibition of its interaction with IRF3, due to HCV NS3 binding or BDV P protein seems to be one mechanism of inhibition of the innate immune responses of hepatitis C virus (HCV) infection or Borna disease virus infection respectively.
  • Tissue specificity

    Ubiquitous with higher expression in testis.
  • Sequence similarities

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
    Contains 1 protein kinase domain.
  • Cellular localization

    Cytoplasm.
  • Information by UniProt
  • Database links

  • Alternative names

    • EC 2.7.11.1 antibody
    • FLJ11330 antibody
    • FTDALS4 antibody
    • NAK antibody
    • NF kappa B activating kinase antibody
    • NF kB activating kinase antibody
    • NF-kappa-B-activating kinase antibody
    • Serine/threonine protein kinase TBK 1 antibody
    • Serine/threonine protein kinase TBK1 antibody
    • Serine/threonine-protein kinase TBK1 antibody
    • T2K antibody
    • TANK binding kinase 1 antibody
    • TANK-binding kinase 1 antibody
    • TBK 1 antibody
    • Tbk1 antibody
    • TBK1_HUMAN antibody
    see all

Images

  • Dot blot analysis of NAK/TBK1 (phospho S172) phospho peptide (Lane 1) and NAK/TBK1 non-phospho peptide (Lane 2) labelling NAK/TBK1 (phospho S172) phospho peptide with ab109272 at a dilution of 1:1000 dilution (0.627μg/ml). A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1:20,000 dilution.

    Blocking buffer: 5% NFDM/TBST.
    Dilution buffer: 5% NFDM /TBST .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).

  • All lanes : Anti-NAK/TBK1 (phospho S172) antibody [EPR2867(2)] (ab109272) at 1/5000 dilution

    Lane 1 : Untreated NIH/3T3 (mouse embryo) whole cell lysate
    Lane 2 : NIH/3T3 (mouse embryo) treated with 10mM pervanadate for 30 minutes whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 84 kDa



    Blocking buffer and diluting buffer: 5% NFDM /TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109272).

References

ab186469 has not yet been referenced specifically in any publications.

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