Key features and details
- Rabbit polyclonal to NALP2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-NALP2 antibody
See all NALP2 primary antibodies
DescriptionRabbit polyclonal to NALP2
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide corresponding to 12 amino acids near the carboxy-terminus of human NALP2.
- PC-3 cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab36850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 96 kDa (predicted molecular weight: 121 kDa).|
FunctionSuppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases.
Tissue specificityExpressed at high levels in lung, placenta and thymus and at lower levels in ovary, intestine and brain.
Sequence similaritiesBelongs to the NLRP family.
Contains 1 DAPIN domain.
Contains 8 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
DomainThe DAPIN domain is necessary and sufficient for suppression of NFKB1 activation induced by TNF and for inducing IL1B secretion in collaboration with caspase-1. It is involved in interaction with PYCARD.
When isolated, the NACHT domain is involved in interaction with CARD8. This interaction is not detected for the full-length protein, maybe due to autoinhibition, this inhibition might by relieved by an inducible change in protein folding.
- Information by UniProt
- CLR19.9 antibody
- FLJ20510 antibody
- Leucine rich repeat and PYD containing protein 2 antibody
Anti-NALP2 antibody (ab36850) at 1 µg/ml + PC-3 cell lysate
Predicted band size: 121 kDa
Observed band size: 96 kDa why is the actual band size different from the predicted?
ab36850 has been referenced in 2 publications.
- Brand FJ et al. Acidification changes affect the inflammasome in human nucleus pulposus cells. J Inflamm (Lond) 13:29 (2016). Human . PubMed: 27563282
- Ji S et al. Toll-like receptor 2 and NALP2 mediate induction of human beta-defensins by fusobacterium nucleatum in gingival epithelial cells. Infect Immun 77:1044-52 (2009). WB ; Human . PubMed: 19103770