Key features and details
- Goat polyclonal to NALP5 - C-terminal
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab215180 was purified from goat serum by ammonium sulphate precipitation followed by immunogen affinity chromatography.
Our Abpromise guarantee covers the use of ab215180 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 130 kDa (predicted molecular weight: 134 kDa).
1 hour primary incubation is recommended for this product.
FunctionAs a member of the subcortical maternal complex (SCMC), plays an essential role for zygotes to progress beyond the first embryonic cell divisions.
Sequence similaritiesBelongs to the NLRP family.
Contains 13 LRR (leucine-rich) repeats.
Contains 1 NACHT domain.
Contains 1 pyrin domain.
Cellular localizationMitochondrion. Nucleus, nucleolus. Cytoplasm. In the subcortical cytoplasm of early embryos from the 1-cell to the blastocyst stages. From the 2-cell stage, still detected in the subcortex, but excluded from cell-cell contact regions (By similarity).
- Information by UniProt
- CLR19.8 antibody
- MATER antibody
- Mater protein homolog antibody
Anti-NALP5 antibody - C-terminal (ab215180) at 2 µg/ml + Human ovary lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 134 kDa
Observed band size: ~130 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour.
ab215180 has not yet been referenced specifically in any publications.