Overview

  • Product name
    Anti-Nanog antibody [23D2-3C6]
    See all Nanog primary antibodies
  • Description
    Mouse monoclonal [23D2-3C6] to Nanog
  • Host species
    Mouse
  • Specificity
    ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey
  • Immunogen

    Recombinant full length protein corresponding to Human Nanog aa 1-305. expressed in bacteria.
    Sequence:

    MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV


    Database link: Q9H9S0

  • Positive control
    • NTERA-2 cells; HEL 11.4 induced IPS cells; H9 embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab173368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100.

Target

  • Function
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Images

  • Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green)  followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1 : NCCIT nuclear fraction lysate
    Lane 2 : NCCIT cytoplasmic fraction lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    4-20% Tris-HCl polyacrylamide gel.

  • All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

    Lane 1 : NCCIT lysate
    Lane 2 : NTERA-2 lysate
    Lane 3 : HeLa lysate

    Lysates/proteins at 60 µg per lane.

    Secondary
    All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 34 kDa



    4-20% Tris-HCl polyacrylamide gel

  • Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution. 

  • Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram)  followed by a fluorescein-conjugated secondary antibody at 1/200 dilution. 

  • Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green)  followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.

  • Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

References

This product has been referenced in:
  • Fang D  et al. Mfn2 is Required for Mitochondrial Development and Synapse Formation in Human Induced Pluripotent Stem Cells/hiPSC Derived Cortical Neurons. Sci Rep 6:31462 (2016). Read more (PubMed: 27535796) »
See 1 Publication for this product

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