Name: Anti-Nanog antibody [23D2-3C6]
SKU: ab173368
Rating: 5 (2 reviews)

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Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

Key features and details

Mouse monoclonal [23D2-3C6] to Nanog
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human
Isotype: IgG

Product name

Anti-Nanog antibody [23D2-3C6]
See all Nanog primary antibodies
Description

Mouse monoclonal [23D2-3C6] to Nanog
Host species

Mouse
Specificity

ab173368 detects an ~38 kDa protein in embryonal carcinoma cells. Subcellular fractionation shows nuclear localization of Nanog. It shows specificity to Nanog and is non-reactive to lysates from non-embryonal cell types (e.g. HeLa cell lysate).
Tested applications

Suitable for: WB, ICC/IF, Flow Cytmore details
Species reactivity

Reacts with: Human
Predicted to work with: Chimpanzee, Cynomolgus monkey
Immunogen

Recombinant full length protein corresponding to Human Nanog aa 1-305. expressed in bacteria.
Sequence:
MSVDPACPQS LPCFEASDCK ESSPMPVICG PEENYPSLQM SSAEMPHTET VSPLPSSMDL LIQDSPDSST SPKGKQPTSA EKSVAKKEDK VPVKKQKTRT VFSSTQLCVL NDRFQRQKYL SLQQMQELSN ILNLSYKQVK TWFQNQRMKS KRWQKNNWPK NSNGVTQKAS APTYPSLYSS YHQGCLVNPT GNLPMWSNQT WNNSTWSNQT QNIQSWSNHS WNTQTWCTQS WNNQAWNSPF YNCGEESLQS CMQFQPNSPA SDLEAALEAA GEGLNVIQQT TRYFSTPQTM DLFLNYSMNM QPEDV

Database link: Q9H9S0
Run BLAST with Run BLAST with
Positive control
- NTERA-2 cells; HEL 11.4 induced IPS cells; H9 embryonic stem cells. NCCIT cells.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.05% Sodium azide
Constituents: 30% Glycerol, 0.1% BSA, 69% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

23D2-3C6
Isotype

IgG
Research areas

Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (HRP) (ab205719)
Isotype control
- Mouse IgG - Isotype Control (ab37355)
Recombinant Protein
- Recombinant human Nanog protein (ab134886)
Related Products
- Recombinant human Nanog protein (ab134886)
- Recombinant Human Nanog protein (ab50053)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab173368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/500 - 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF	(1)	1/50 - 1/100.
Flow Cyt		1/100.

Notes
WB 1/500 - 1/2000. Predicted molecular weight: 34 kDa.
ICC/IF 1/50 - 1/100.
Flow Cyt 1/100.

Function

Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificity

Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similarities

Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stage

Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
Cellular localization

Nucleus.
Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
- hNanog antibody
- Homeobox protein NANOG antibody
- Homeobox transcription factor Nanog antibody
- homeobox transcription factor Nanog-delta 48 antibody
- NANOG antibody
- Nanog homeobox antibody
- NANOG_HUMAN antibody
see all

Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NTERA-2 cell line
Lane 2 : NCCIT cell line
Lane 3 : iPSC cell line
Lane 4 : iPSC differentiated to definitive endoderm
Lane 5 : HeLa cell line

Lysates/proteins at 30 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/40000 dilution

Predicted band size: 34 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of H9 embryonic stem cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.
Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NCCIT nuclear fraction lysate
Lane 2 : NCCIT cytoplasmic fraction lysate

Lysates/proteins at 60 µg per lane.

Secondary
All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa

4-20% Tris-HCl polyacrylamide gel.
Western blot - Anti-Nanog antibody [23D2-3C6] (ab173368)

All lanes : Anti-Nanog antibody [23D2-3C6] (ab173368) at 1/1000 dilution

Lane 1 : NCCIT lysate
Lane 2 : NTERA-2 lysate
Lane 3 : HeLa lysate

Lysates/proteins at 60 µg per lane.

Secondary
All lanes : goat anti-mouse IgG-HRP at 1/20000 dilution

Developed using the ECL technique.

Predicted band size: 34 kDa

4-20% Tris-HCl polyacrylamide gel
Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

Flow cytometric analysis of H9 embryonic stem cells labeling Nanog with ab173368 at 1/100 (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary at 1/200 dilution.
Flow Cytometry - Anti-Nanog antibody [23D2-3C6] (ab173368)

Flow cytometric analysis of HEL 11.4 induced IPS cells labeling Nanog with ab173368 at 1/00 dilution (blue histogram) or mouse IgG (green histogram) followed by a fluorescein-conjugated secondary antibody at 1/200 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of HEL 11.4 induced IPS cells grown for a few days on Matrigel-coated chamber slides (4% paraformaldehyde fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/200 dilution (green) followed by a fluorescein-conjugated secondary antibody at 1/100 dilution. Nuclei (blue) were stained with DAPI.
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody [23D2-3C6] (ab173368)

Immunofluorescent analysis of NTERA-2 and HeLa cells (formalin-fixed and permeabilized with 0.1% Triton X-100) labeling Nanog with ab173368 at 1/50 dilution (green) followed by DyLight 488-conjugated goat anti-mouse IgG secondary antibody. F-Actin (red) was stained with DyLight-554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye.

Flow cytometry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab173368? Please let us know so that we can cite the reference in this datasheet.

ab173368 has been referenced in 1 publication.

Fang D et al. Mfn2 is Required for Mitochondrial Development and Synapse Formation in Human Induced Pluripotent Stem Cells/hiPSC Derived Cortical Neurons. Sci Rep 6:31462 (2016). PubMed: 27535796

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (hiPSC)

Permeabilization

Yes - PBS + 0.1% Triton X-100

Specification

hiPSC

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Paraformaldehyde

Dr. Gabriel Galea

Verified customer

Submitted Feb 05 2021

Application

IHC - Wholemount

Sample

Mouse Embryo (Pharyngeal arches)

Specification

Pharyngeal arches

Dr. Gabriel Galea

Verified customer

Submitted Dec 15 2020

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Key features and details

Overview

Product name

Description

Host species

Specificity

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Compatible Secondaries

Isotype control

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Sequence similarities

Developmental stage

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (1)

Customer reviews and Q&As

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Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody [23D2-3C6]

IHC - Wholemount abreview for Anti-Nanog antibody [23D2-3C6]