Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Nanog antibody
See all Nanog primary antibodies
DescriptionRabbit polyclonal to Nanog
SpecificityAt least two isoforms of Nanog are known to exist; ab106465 will detect both.
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
A 19 amino acid synthetic peptide near the center of Human Nanog (EAW88651).
- Human spleen tissue lysate; Human spleen tissue
Storage instructionsShipped at 4°C. Store at 4°C (stable for up to 12 months).
Storage bufferPreservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab106465 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 µg/ml.|
|ICC/IF||Use a concentration of 20 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.|
FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similaritiesBelongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
- Information by UniProt
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.
Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/ml
Lane 2 : Anti-Nanog antibody (ab106465) at 2 µg/ml
All lanes : Human spleen tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 35 kDa
ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.
ab106465 has been referenced in 5 publications.
- Guo JC et al. Silencing of long noncoding RNA HOXA11-AS inhibits the Wnt signaling pathway via the upregulation of HOXA11 and thereby inhibits the proliferation, invasion, and self-renewal of hepatocellular carcinoma stem cells. Exp Mol Med 51:1-20 (2019). PubMed: 31757938
- Li H et al. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell Stem Cell 20:462-477.e6 (2017). PubMed: 28111200
- Zhang J et al. Moderate treadmill running exercise prior to tendon injury enhances wound healing in aging rats. Oncotarget 7:8498-512 (2016). ICC . PubMed: 26885754
- Guan Y et al. The effective bioengineering method of implantation decellularized renal extracellular matrix scaffolds. Oncotarget 6:36126-38 (2015). PubMed: 26418881
- Anchan RM et al. Pax6- and Six3-mediated induction of lens cell fate in mouse and human ES cells. PLoS One 9:e115106 (2014). ICC/IF ; Mouse . PubMed: 25517354