Overview

  • Product name

  • Description

    Rabbit polyclonal to Nanog
  • Host species

    Rabbit
  • Specificity

    At least two isoforms of Nanog are known to exist; ab106465 will detect both.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A 19 amino acid synthetic peptide near the center of Human Nanog (EAW88651).

  • Positive control

    • Human spleen tissue lysate; Human spleen tissue

Properties

Applications

Our Abpromise guarantee covers the use of ab106465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 20 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Images

  • Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.

  • Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/ml
    Lane 2 : Anti-Nanog antibody (ab106465) at 2 µg/ml

    All lanes : Human spleen tissue lysate

    Lysates/proteins at 15 µg per lane.

    Predicted band size: 35 kDa

  • ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.

References

This product has been referenced in:

  • Li H  et al. RNA Helicase DDX5 Inhibits Reprogramming to Pluripotency by miRNA-Based Repression of RYBP and its PRC1-Dependent and -Independent Functions. Cell Stem Cell 20:462-477.e6 (2017). Read more (PubMed: 28111200) »
  • Zhang J  et al. Moderate treadmill running exercise prior to tendon injury enhances wound healing in aging rats. Oncotarget 7:8498-512 (2016). ICC . Read more (PubMed: 26885754) »
See all 4 Publications for this product

Customer reviews and Q&As

Answer

I am sorry to hear that you have been experiencing problems with this antibody. The antibody was delivered on Friday and was received by you on Monday. Now not storing the products as recommended on the datasheet actually voids the Abpromise guarantee and we do believe that this was not an Abcam mistake. So technical we are not liable for replacement. We however have tested data of more than 1000 antibodies which shows the antibodies do not loose activity for about a week at RT. So In order to help you further in your experiment and you being a loyal Abcam customer we have decided to make an exception this time. We are sending you a free of charge replacement for this product. I do however would like to suggest that you should update the person who receives products from Abcam. They should be storing the products at 4C or as recommended on the datasheet. The order number for FOCR is 950162. I hope this will be helpful. Should you have any other question please do not hesitate to contact me.

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