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Anti-Nanog antibody (ab106465)

Submit a review Q&A (1)References (13)
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab106465)
  • Western blot - Anti-Nanog antibody (ab106465)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)

Key features and details

  • Rabbit polyclonal to Nanog
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

Get better batch-to-batch reproducibility with a recombinant antibody

Product image
Anti-Nanog antibody [EPR2027(2)] (ab109250)
  • Research with confidence – consistent and reproducible results with every batch
  • Long-term and scalable supply – powered by recombinant technology for fast production
  • Success from the first experiment – confirmed specificity through extensive validation
  • Ethical standards compliant – production is animal-free

Overview

  • Product name

    Anti-Nanog antibody
    See all Nanog primary antibodies

  • Description

    Rabbit polyclonal to Nanog

  • Host species

    Rabbit

  • Specificity

    At least two isoforms of Nanog are known to exist; ab106465 will detect both.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    A 19 amino acid synthetic peptide near the center of Human Nanog (EAW88651).

  • Positive control

    • Human spleen tissue lysate; Human spleen tissue

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab106465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.
Notes
IHC-P
Use a concentration of 5 µg/ml.
ICC/IF
Use a concentration of 20 µg/ml.
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 35 kDa.

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.

  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.

  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.

  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab106465)
      Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab106465)
      Immunofluorescence of NANOG in Human Spleen cells using ab106465 at 20 ug/ml.

    • Western blot - Anti-Nanog antibody (ab106465)
      Western blot - Anti-Nanog antibody (ab106465)
      Lane 1 : Anti-Nanog antibody (ab106465) at 1 µg/ml
      Lane 2 : Anti-Nanog antibody (ab106465) at 2 µg/ml

      All lanes : Human spleen tissue lysate

      Lysates/proteins at 15 µg per lane.

      Predicted band size: 35 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab106465)
      ab106465, at 5 µg/ml, staining Nanog in Human spleen tissue by Immunohistochemistry.

    References (13)

    Publishing research using ab106465? Please let us know so that we can cite the reference in this datasheet.

    ab106465 has been referenced in 13 publications.

    • Zhang D  et al. Senolytic controls bone marrow mesenchymal stem cells fate improving bone formation. Am J Transl Res 12:3078-3088 (2020). PubMed: 32655832
    • Zhang Y  et al. Activation of PGK1 under hypoxic conditions promotes glycolysis and increases stem cell-like properties and the epithelial-mesenchymal transition in oral squamous cell carcinoma cells via the AKT signalling pathway. Int J Oncol 57:743-755 (2020). PubMed: 32705252
    • Jeong YM  et al. Substance P enhances the local activation of NK1R-expressing c-kit+ cardiac progenitor cells in right atrium of ischemia/reperfusion-injured heart. BMC Mol Cell Biol 21:41 (2020). PubMed: 32517655
    • Tang D  et al. Transfer of metastatic traits via miR-200c in extracellular vesicles derived from colorectal cancer stem cells is inhibited by atractylenolide I. Clin Transl Med 10:e139 (2020). PubMed: 32898324
    • Wang L  et al. Establishment of a feeder and serum-free culture system for human embryonic stem cells. Zygote 28:175-182 (2020). PubMed: 31965957
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    Question

    Antibody received Monday; did not store it at 4C as recommended on the datasheet. - Failed in IHC-P - Rat embryonic tissue lysates - Tried at 5ug/ml and also at other dilutions. - No staining at all.

    Read More
    Answer

    I am sorry to hear that you have been experiencing problems with this antibody. The antibody was delivered on Friday and was received by you on Monday. Now not storing the products as recommended on the datasheet actually voids the Abpromise guarantee and we do believe that this was not an Abcam mistake. So technical we are not liable for replacement. We however have tested data of more than 1000 antibodies which shows the antibodies do not loose activity for about a week at RT. So In order to help you further in your experiment and you being a loyal Abcam customer we have decided to make an exception this time. We are sending you a free of charge replacement for this product. I do however would like to suggest that you should update the person who receives products from Abcam. They should be storing the products at 4C or as recommended on the datasheet. The order number for FOCR is 950162. I hope this will be helpful. Should you have any other question please do not hesitate to contact me.

    Read More

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