Anti-Nanog antibody (ab14959)
- Datasheet
- References (9)
- Protocols
Overview
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Product nameAnti-Nanog antibody
See all Nanog primary antibodies -
DescriptionRabbit polyclonal to Nanog
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-Pmore details
Unsuitable for: ICC/IF -
Species reactivityReacts with: Mouse
Does not react with: Human -
Immunogen
Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog.
Read Abcam's proprietary immunogen policy . -
Positive control
- This antibody gave a positive signal in the following whole cell lysates: Mouse Embryonic Stem Cell.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab14959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). | |
| IHC-P | 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
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Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
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Sequence similaritiesBelongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 71950 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Western blot - Anti-Nanog antibody (ab14959)All lanes : Anti-Nanog antibody (ab14959) at 1 µg/ml
Lanes 3-4 : Mouse Nanog peptide (ab14960) at 1 µg/ml
Predicted band size: 34 kDa
Observed band size: 34.6 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity)Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml
Observed band size: 34.2 kDa
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
Lanes 2 and 4: Human embryonic stem cell lysate (negative control)ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.
Performed under reducing conditions.
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Western blot - Anti-Nanog antibody (ab14959)This image is courtesy of Adam Yates and Ian Chambers, University of EdinburghAll lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml
All lanes : ES Cell Nuclear Extract
Predicted band size: 34 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Nanog antibody (ab14959)This image is courtesy of an Abreview submitted by Carl Hobbsab14959 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab14959 at 1/600 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.
Datasheets and documents
References
This product has been referenced in:
- Kim TW et al. Ctbp2-mediated ß-catenin regulation is required for exit from pluripotency. Exp Mol Med 49:e385 (2017). Read more (PubMed: 29026198) »
- Shin J et al. Aurkb/PP1-mediated resetting of Oct4 during the cell cycle determines the identity of embryonic stem cells. Elife 5:e10877 (2016). Read more (PubMed: 26880562) »
