Anti-Nanog antibody (ab14959)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
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- Success from the first experiment – confirmed specificity through extensive validation
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Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Rabbit polyclonal to Nanog -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Mouse Nanog aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab14960) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab14959 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
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| Notes |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa). |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Entrez Gene: 71950 Mouse
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- SwissProt: Q80Z64 Mouse
- Unigene: 635882 Human
- Unigene: 6047 Mouse
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Western blot - Anti-Nanog antibody (ab14959)All lanes : Anti-Nanog antibody (ab14959) at 1 µg/ml
Lanes 3-4 : Mouse Nanog peptide (ab14960) at 1 µg/ml
Predicted band size: 34 kDa
Observed band size: 34.6 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity)Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml
Observed band size: 34.2 kDa
Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
Lanes 2 and 4: Human embryonic stem cell lysate (negative control)ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.
Performed under reducing conditions.
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Western blot - Anti-Nanog antibody (ab14959)This image is courtesy of Adam Yates and Ian Chambers, University of EdinburghAll lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml
All lanes : ES Cell Nuclear Extract
Predicted band size: 34 kDa
Datasheets and documents
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Datasheet download
References (17)
ab14959 has been referenced in 17 publications.
- Heo J et al. Phosphorylation of TFCP2L1 by CDK1 is required for stem cell pluripotency and bladder carcinogenesis. EMBO Mol Med 12:e10880 (2020). PubMed: 31709755
- Belair DG et al. Thalidomide Inhibits Human iPSC Mesendoderm Differentiation by Modulating CRBN-dependent Degradation of SALL4. Sci Rep 10:2864 (2020). PubMed: 32071327
- Dong H et al. GLI1 activation by non-classical pathway integrin avß3/ERK1/2 maintains stem cell-like phenotype of multicellular aggregates in gastric cancer peritoneal metastasis. Cell Death Dis 10:574 (2019). WB ; Human . PubMed: 31366904
- Kim HJ et al. Cyclin-dependent kinase 1 activity coordinates the chromatin associated state of Oct4 during cell cycle in embryonic stem cells. Nucleic Acids Res 46:6544-6560 (2018). PubMed: 29901724
- Suh MY et al. Abundance of C-terminal binding protein isoform is a prerequisite for exit from pluripotency in mouse embryonic stem cells. FASEB J N/A:fj201700837RRRR (2018). PubMed: 29894668
