• Product name
  • Description
    Rabbit polyclonal to Nanog
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    Synthetic peptide derived from residues 250 to the C-terminus of Mouse Nanog.

    Read Abcam's proprietary immunogen policy .

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Mouse Embryonic Stem Cell.



Our Abpromise guarantee covers the use of ab14959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 34 kDa (predicted molecular weight: 34 kDa).
IHC-P 1/600. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function
      Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • Tissue specificity
      Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • Sequence similarities
      Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage
      Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • Cellular localization
    • Information by UniProt
    • Database links
    • Alternative names
      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all


    • All lanes : Anti-Nanog antibody (ab14959) at 1 µg/ml

      Lanes 3-4 : Mouse Nanog peptide (ab14960) at 1 µg/ml

      Predicted band size: 34 kDa
      Observed band size: 34.6 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity)

      Lanes 1-4 : Nanog antibody (ab14959) at 1 ug/ml
      Lanes 3-4 : Nanog peptide (ab14960) at 1 ug/ml

      Observed band size: 34.2 kDa
      Additional bands at: 18 kDa (possible cross reactivity), 38 kDa (possible cross reactivity), 50 kDa (possible cross reactivity).

      Lanes 1 and 3: Mouse embryonic stem cell lysate (positive control)
      Lanes 2 and 4: Human embryonic stem cell lysate (negative control)

      ab14959 recognised a band of the expected size for Nanog in mouse embryonic stem cells but not in human embryonic stem cells. Additional bands were also recognised in mouse embyonic stem cells.

      Performed under reducing conditions.

    • All lanes : Anti-Nanog antibody (ab14959) at 0.8 µg/ml

      All lanes : ES Cell Nuclear Extract

      Predicted band size: 34 kDa

    • ab14959 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab14959 at 1/600 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

      See Abreview


    This product has been referenced in:
    • Kim TW  et al. Ctbp2-mediated ß-catenin regulation is required for exit from pluripotency. Exp Mol Med 49:e385 (2017). Read more (PubMed: 29026198) »
    • Shin J  et al. Aurkb/PP1-mediated resetting of Oct4 during the cell cycle determines the identity of embryonic stem cells. Elife 5:e10877 (2016). Read more (PubMed: 26880562) »
    See all 9 Publications for this product

    Customer reviews and Q&As

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    1-2 of 2 Abreviews

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Mouse Tissue sections (Teratoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Apr 12 2012

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Cell lysate - whole cell (ES cell)
    Loading amount
    10 µg
    ES cell
    2000 U/ml mouse LIF constantly
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

    Abcam user community

    Verified customer

    Submitted Feb 12 2009


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