Anti-Nanog antibody (ab70482)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
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Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Rabbit polyclonal to Nanog -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70482 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (2) |
1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
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| Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa). |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 71950 Mouse
- SwissProt: Q80Z64 Mouse
- Unigene: 6047 Mouse
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Western blot - Anti-Nanog antibody (ab70482)All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml
Lane 1 : Whole cell lysate
from mouse embryonic stem cells at 15 µg
Lane 2 : Whole cell lysate
from mouse embryonic stem cells at 50 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 39 kDa why is the actual band size different from the predicted?
Exposure time: 12 seconds -
Western blot - Anti-Nanog antibody (ab70482)This image is courtesy of an anonymous AbreviewAll lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)
Lane 1 : Murine Embryonic Fibroblasts (MEFs)
Lane 2 : Murine Embryonic Stem (ES) Cells
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Blocking Step: 3% BSA for 1 hour at 20°C
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab70482 has been referenced in 8 publications.
- Dong W et al. SATB2 knockdown decreases hypoxia-induced autophagy and stemness in oral squamous cell carcinoma. Oncol Lett 20:794-802 (2020). PubMed: 32566006
- Lin S et al. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71:244-255.e5 (2018). PubMed: 29983320
- Du P et al. An Intermediate Pluripotent State Controlled by MicroRNAs Is Required for the Naive-to-Primed Stem Cell Transition. Cell Stem Cell 22:851-864.e5 (2018). PubMed: 29804889
- Fritz AL et al. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming. Mol Ther 23:952-963 (2015). PubMed: 25666918
- O'Reilly LP et al. An unexpected role for the clock protein timeless in developmental apoptosis. PLoS One 6:e17157 (2011). WB ; Mouse . PubMed: 21359199
- Kinney SM et al. Tissue-specific distribution and dynamic changes of 5-hydroxymethylcytosine in mammalian genomes. J Biol Chem 286:24685-93 (2011). WB ; Mouse . PubMed: 21610077
- Fagoonee S et al. Generation of functional hepatocytes from mouse germ line cell-derived pluripotent stem cells in vitro. Stem Cells Dev 19:1183-94 (2010). ICC/IF ; Mouse . PubMed: 20331356
- Quinlan MP et al. Activated Kras, but not Hras or Nras, may initiate tumors of endodermal origin via stem cell expansion. Mol Cell Biol 28:2659-74 (2008). WB ; Human . PubMed: 18268007