Overview

  • Product name

  • Description

    Rabbit polyclonal to Nanog
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WB, IPmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    A region between residue 250 and the C-terminus (residue 305) of Mouse Nanog using the numbering given in entry NP_079141.2

  • Positive control

    • Whole cell lysate from mouse embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab70482 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20331356
IHC-P 1/750. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
IP Use at 2-5 µg/mg of lysate.

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Images

  • All lanes : Anti-Nanog antibody (ab70482) at 0.1 µg/ml

    Lane 1 : Whole cell lysate
    from mouse embryonic stem cells at 15 µg
    Lane 2 : Whole cell lysate
    from mouse embryonic stem cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 39 kDa
    why is the actual band size different from the predicted?


    Exposure time: 12 seconds
  • ab70482 staining Nanog in murine embryonic stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in paraformaldehyde, permeabilised in 0.05% Triton X-100 and blocked using 10% serum for 2 hours at 20°C. Samples were then incubated with primary antibody at 1/500 for 16 hours at 4°C. The secondary antibody used was a goat anti-rabbit IgG (H+L) conjugated to Alexa Fluor® 488 (green) used at a 1/1000 dilution.

    See Abreview

  • Detection of Mouse Nanog by Western Blot of Immunoprecipitation with whole cell lysate (1mg) from mouse embryonic stem cells and ab 70482 at 2µg/mg lysate. Lane 1: ab70482, Lane 2: rabbit IgG control Immunoprecipitated Nanog was blotted using an anti-Nanog antibody at 0.1µg/ml.
  • All lanes : Anti-Nanog antibody (ab70482) at 1/1000 dilution (for 16 hours at 4°C)

    Lane 1 : Murine Embryonic Fibroblasts (MEFs)
    Lane 2 : Murine Embryonic Stem (ES) Cells

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : An HRP-conjugated Donkey anti-rabbit IgG monoclonal at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa why is the actual band size different from the predicted?


    Exposure time: 1 minute


    Blocking Step: 3% BSA for 1 hour at 20°C

    See Abreview

  • ab70482 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with HIER buffer. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab70482 at 1/750 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Lin S  et al. Mettl1/Wdr4-Mediated m7G tRNA Methylome Is Required for Normal mRNA Translation and Embryonic Stem Cell Self-Renewal and Differentiation. Mol Cell 71:244-255.e5 (2018). Read more (PubMed: 29983320) »
  • Fritz AL  et al. cAMP and EPAC Signaling Functionally Replace OCT4 During Induced Pluripotent Stem Cell Reprogramming. Mol Ther 23:952-963 (2015). Read more (PubMed: 25666918) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer



The immunogen used to produce ab70482 is based on the mouse nanog sequence. The exact immunogen sequence is proprietary however.

Read More

Answer

Thank you for contacting us.

1. The alternative to Alexa Fluor® 488 goat anti-mouse Ig Cat # G A-11001 is ab96871; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-ab96871.html

or ab96879; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-488-pre-adsorbed-ab96879.html

2. ab96895; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-DyLight-488-pre-adsorbed-ab96895.html

3. ab97035; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-Cy3-pre-adsorbed-ab97035.html

4. ab97075; https://www.abcam.com/Goat-polyclonal-Secondary-Antibody-to-Rabbit-IgG-H-L-Cy3-pre-adsorbed-ab97075.html

5. ab10345; https://www.abcam.com/PSTAIR-antibody-PSTAIR-ab10345.html

6. We do not have anti Nanog mouse monoclonal that customer is after. We have similar rabbit polyclonal antibodies available. The links are

https://www.abcam.com/Nanog-antibody-ab70482.html

https://www.abcam.com/Nanog-antibody-ab90640.html

7. ab109187; https://www.abcam.com/Oct4-antibody-EPR2053-2-ab109187.html

8. ab97959; https://www.abcam.com/SOX2-antibody-ab97959.html

9. ab131000; https://www.abcam.com/Caspase-3-antibody-ab131000.html

10. ab18737; https://www.abcam.com/Caspase-2-antibody-ab18737.html

11. We do not sell this chemical.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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