Western blot abreview for Anti-Nanog antibody

Excellent
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Embryonic)
Loading amount
50 µg
Specification
Embryonic
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: ECL Rabbit Whole Molecule
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 40 kDa
Positive control
Lane 2: Murine Embryonic Stem (ES) Cells
Negative control
Lane 1: Murine Embryonic Fibroblasts (MEFs)

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Submitted Jul 20 2010

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