Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Specification
Embryonic
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.05% Triton X added to blocking solution
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Goat Serum, 0.005% Triton-X in PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 goat anti-rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Image depicts mouse ES colony grown on irradiated MEFs, fixed with 4% PFA.
Blocked in 10% Normal Goat Serum, 0.05% Triton X-100 in PBS.

Signal observed in ES colonies and not in feeder monolayer.

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Submitted Mar 18 2011

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