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Mouse Cell (Embryonic)
Yes - 0.05% Triton X added to blocking solution
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
Other product details
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% Goat Serum, 0.005% Triton-X in PBS
Non-Abcam antibody was used: Alexa Fluor® 488 goat anti-rabbit IgG (H+L)
Host species: Goat
Conjugation: Alexa Fluor® 488
Image depicts mouse ES colony grown on irradiated MEFs, fixed with 4% PFA.
Blocked in 10% Normal Goat Serum, 0.05% Triton X-100 in PBS.
Signal observed in ES colonies and not in feeder monolayer.
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Submitted Mar 18 2011