Anti-Nanog antibody (ab77095)
Key features and details
- Goat polyclonal to Nanog
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Goat polyclonal to Nanog -
Host species
Goat -
Specificity
We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig
Does not react with:
Mouse -
Immunogen
Synthetic peptide:
QNQRMKSKRWQKNN
by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog -
Positive control
- Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab77095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).
A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
| Notes |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples. |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Western blot - Anti-Nanog antibody (ab77095)Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab77095 has been referenced in 5 publications.
- Li Q et al. Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. Cell Reprogram 22:82-89 (2020). PubMed: 32125888
- Chailangkarn T et al. A human neurodevelopmental model for Williams syndrome. Nature 536:338-43 (2016). PubMed: 27509850
- Lim J et al. Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors. Sci Rep 4:4361 (2014). ICC/IF ; Human . PubMed: 24618595
- Nishimura T et al. Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells. Stem Cells Dev N/A:N/A (2013). PubMed: 23409943
- Montserrat N et al. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system. J Biol Chem 286:12417-28 (2011). ICC/IF . PubMed: 21285354