Overview

  • Product name

  • Description

    Goat polyclonal to Nanog
  • Host species

    Goat
  • Specificity

    We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species.
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog, PigDoes not react with: Mouse
  • Immunogen

    Synthetic peptide:

    QNQRMKSKRWQKNN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog

  • Positive control

    • Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

ab77095 is not suitable for Western Blot with pig samples.

ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Images

  • Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with ab77095 at 5µg/ml (green staining).

References

This product has been referenced in:

See all 4 Publications for this product

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