Anti-Nanog antibody (ab77095)
- Datasheet
- References (4)
- Protocols
Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Goat polyclonal to Nanog -
Host species
Goat -
Specificity
We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Pig
Does not react with:
Mouse -
Immunogen
Synthetic peptide:
QNQRMKSKRWQKNN
by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog -
Positive control
- Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab77095 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa). ab77095 is not suitable for Western Blot with pig samples. |
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| ICC/IF | Use a concentration of 5 µg/ml. |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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Western blot - Anti-Nanog antibody (ab77095)Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg
Developed using the ECL technique.
Predicted band size: 35 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab77095)Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with ab77095 at 5µg/ml (green staining).
References
This product has been referenced in:
- Chailangkarn T et al. A human neurodevelopmental model for Williams syndrome. Nature 536:338-43 (2016). Read more (PubMed: 27509850) »
- Lim J et al. Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors. Sci Rep 4:4361 (2014). ICC/IF ; Human . Read more (PubMed: 24618595) »
