Overview

  • Product name
    Anti-Nanog antibody
    See all Nanog primary antibodies
  • Description
    Goat polyclonal to Nanog
  • Host species
    Goat
  • Specificity
    We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species.
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Dog, PigDoes not react with: Mouse
  • Immunogen

    Synthetic peptide:

    QNQRMKSKRWQKNN

    by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab77095 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

ab77095 is not suitable for Western Blot with pig samples.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
    • Alternative names
      • Embryonic stem cell specific homeobox protein (Nanog) antibody
      • ENK antibody
      • FLJ12581 antibody
      • hNanog antibody
      • Homeobox protein NANOG antibody
      • Homeobox transcription factor Nanog antibody
      • homeobox transcription factor Nanog-delta 48 antibody
      • NANOG antibody
      • Nanog homeobox antibody
      • NANOG_HUMAN antibody
      see all

    Images

    • Western blot - Anti-Nanog antibody (ab77095)
      Western blot - Anti-Nanog antibody (ab77095)
      Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg

      Developed using the ECL technique.

      Predicted band size: 35 kDa
      Observed band size: 38 kDa (why is the actual band size different from the predicted?)

    • Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab77095)
      Immunocytochemistry/ Immunofluorescence - Anti-Nanog antibody (ab77095)
      Immunofluorescence staining of a colony of induced pluriform stem cells derived from human keratinocytes labelled with ab77095 at 5µg/ml (green staining).

    References

    This product has been referenced in:
    • Lim J  et al. Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors. Sci Rep 4:4361 (2014). ICC/IF ; Human . Read more (PubMed: 24618595) »
    • Nishimura T  et al. Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells. Stem Cells Dev N/A:N/A (2013). Read more (PubMed: 23409943) »

    See all 3 Publications for this product

    Publishing research using ab77095? Please let us know so that we can cite the reference in this datasheet.

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