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    nanog-antibody-ab77095.pdf

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Stem Cells Embryonic Stem Cells Intracellular
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Anti-Nanog antibody (ab77095)

  • Datasheet
  • SDS
Submit a review Submit a question References (7)

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Western blot - Anti-Nanog antibody (ab77095)

    Key features and details

    • Goat polyclonal to Nanog
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    Get better batch-to-batch reproducibility with a recombinant antibody

    Product image
    Anti-Nanog antibody [EPR2027(2)] (ab109250)
    • Research with confidence – consistent and reproducible results with every batch
    • Long-term and scalable supply – powered by recombinant technology for fast production
    • Success from the first experiment – confirmed specificity through extensive validation
    • Ethical standards compliant – production is animal-free

    Overview

    • Product name

      Anti-Nanog antibody
      See all Nanog primary antibodies
    • Description

      Goat polyclonal to Nanog
    • Host species

      Goat
    • Specificity

      We have received reports this antibody does not work in pig, therefore we cannot guarantee it will work in this species.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Dog, PigDoes not react with: Mouse
    • Immunogen

      Synthetic peptide:

      QNQRMKSKRWQKNN

      by a Cysteine residue linker, corresponding to internal sequence amino acids 144-157 of Human Nanog
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Induced pluriform stem cells derived from human keratinocytes. Human ovary lysate.
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% BSA, 99% Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Stem Cells
      • Embryonic Stem Cells
      • Intracellular
      • Epigenetics and Nuclear Signaling
      • Nuclear Signaling Pathways
      • SMADs
      • Epigenetics and Nuclear Signaling
      • Transcription
      • Transcription Factors
      • Cancer
      • Cell cycle
      • Cell differentiation
      • Developmental Biology
      • Embryogenesis
      • Embryonic stem cells
      • Surface molecules

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Positive Controls

      • Recombinant Human Nanog protein (ab50053)
    • Recombinant Protein

      • Recombinant human Nanog protein (ab134886)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab77095 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB
    Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

    A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples.

    Notes
    WB
    Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 35 kDa).

    A 1 hour primary incubation is recommended for this product. ab77095 is not suitable for Western Blot with pig samples.

    Target

    • Function

      Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
    • Tissue specificity

      Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
    • Sequence similarities

      Belongs to the Nanog homeobox family.
      Contains 1 homeobox DNA-binding domain.
    • Developmental stage

      Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9H9S0 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 100293888 Human
      • Entrez Gene: 79923 Human
      • Omim: 607937 Human
      • SwissProt: Q9H9S0 Human
      • Unigene: 635882 Human
      • Alternative names

        • Embryonic stem cell specific homeobox protein (Nanog) antibody
        • ENK antibody
        • FLJ12581 antibody
        • hNanog antibody
        • Homeobox protein NANOG antibody
        • Homeobox transcription factor Nanog antibody
        • homeobox transcription factor Nanog-delta 48 antibody
        • NANOG antibody
        • Nanog homeobox antibody
        • NANOG_HUMAN antibody
        see all

      Images

      • Western blot - Anti-Nanog antibody (ab77095)
        Western blot - Anti-Nanog antibody (ab77095)
        Anti-Nanog antibody (ab77095) at 0.1 µg/ml + Human ovary lysate at 35 µg

        Developed using the ECL technique.

        Predicted band size: 35 kDa
        Observed band size: 38 kDa why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (7)

      Publishing research using ab77095? Please let us know so that we can cite the reference in this datasheet.

      ab77095 has been referenced in 7 publications.

      • Trujillo CA  et al. Pharmacological reversal of synaptic and network pathology in human MECP2-KO neurons and cortical organoids. EMBO Mol Med 13:e12523 (2021). PubMed: 33501759
      • Pinto F  et al. The Extracellular Small Leucine-Rich Proteoglycan Biglycan Is a Key Player in Gastric Cancer Aggressiveness. Cancers (Basel) 13:N/A (2021). PubMed: 33809543
      • Li Q  et al. Inhibition of DNA Methyltransferase by RG108 Promotes Pluripotency-Related Character of Porcine Bone Marrow Mesenchymal Stem Cells. Cell Reprogram 22:82-89 (2020). PubMed: 32125888
      • Chailangkarn T  et al. A human neurodevelopmental model for Williams syndrome. Nature 536:338-43 (2016). PubMed: 27509850
      • Lim J  et al. Partial somatic to stem cell transformations induced by cell-permeable reprogramming factors. Sci Rep 4:4361 (2014). ICC/IF ; Human . PubMed: 24618595
      • Nishimura T  et al. Generation of Functional Platelets from Canine Induced Pluripotent Stem Cells. Stem Cells Dev N/A:N/A (2013). PubMed: 23409943
      • Montserrat N  et al. Simple generation of human induced pluripotent stem cells using poly-beta-amino esters as the non-viral gene delivery system. J Biol Chem 286:12417-28 (2011). ICC/IF . PubMed: 21285354

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