Overview

  • Product name
  • Description
    Rabbit polyclonal to Nanog
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC - Wholemount, ICC, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Recombinant full length protein corresponding to Mouse Nanog.

  • Positive control
    • ICC/IF: Mouse embryonic stem cells. WB: Mouse embryonic stem cell line D3. IHC-P: Mouse teratoma tissue. IHC-Wm: Mouse pre-implantation embryo. Flow Cyt: Mouse embryonic stem cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab80892 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IHC - Wholemount Use at an assay dependent concentration.
ICC 1/150 - 1/700.
WB 1/300 - 1/2000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IHC-P 1/400. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function
    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity
    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities
    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage
    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all

Images

  • Spatio-temporal changes in expression of pluripotency markers and self-organization of stem cell-derived aggregates in 3D culture.
    Panels A-D:
    Expression of the pluripotency marker Nanog decreases as vesicles mature between differentiation days 5–8.

    Panels A’-D’: Progression of outer epithelium ruffling indicating self-organization.

    Three mouse embryonic stem cell aggregates were fixed with 4% paraformaldehyde, then cryoprotected with a serial treatment of 15% and 30% sucrose and embedded in tissue freezing medium. Frozen tissue blocks were sectioned into 10 or 12 μm cyrosections. For immunostaining, a 3% goat or horse serum and 0.1% Triton-X100 solution was used for primary antibody incubation. An Alexa Fluor® 488, 568, or 647 conjugated anti-mouse IgG or anti-goat IgG and an Alexa Fluor 568 or 647 conjugated anti-rabbit IgG were used as secondary antibodies. A DAPI counterstain was used to visualize cellular nuclei.

    Microscopy was performed on a Nikon TE2000 Inverted Microscope or an Olympus FV1000-MPE Confocal/Multiphoton Microscope.

  • ab80892 staining Nanog in mouse embryonic stem cells by immunocytochemistry/ immunofluorescence.

    Cells were paraformaldehyde fixed and permeabilized in Tween-20 prior to blocking in 20% serum for 30 minutes at 25°C. The primary antibody was diluted 1/100 and incubated with the sample for 4 hour at 25°C. A Cy®5 conjugated goat polyclonal to rabbit IgG antibody, diluted 1/100, was used as the secondary.

    Nuclear staining with DAPI.

    See Abreview

  • All lanes : Anti-Nanog antibody (ab80892) at 1/500 dilution

    Lane 1 : Marker
    Lanes 2-3 : Mouse embryonic stem cell line D3
    Lanes 4-5 : Mutant mouse embryonic stem cell line known to not express nanog

    Lysates/proteins at 200000 cells per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 minutes


    Blocked with milk for 30 minutes.

    See Abreview

  • ab80892 staining mouse teratoma sections by IHC-P. The tissue was fixed with formaldehyde and a heat mediated antigen retrival step was performed with citric acid pH 6. Blocking of the sample was done with 1% BSA for 10 minutes at 21°C, followed by staining with ab80892 at 1/400 in TBS/BSA/azide for 2h at 21°C. A biotinylated goat anti-rabbit polyclonal antibody at 1/200 was used as the secondary antibody.

    See Abreview

  • IHC-Wholemount image of anti-Nanog antibody (ab80892) staining on a mouse pre-implantation embryo.

    This embryo was fixed in paraformaldehyde, permeabilized in 0.25% Triton X and then blocked in 5% serum for 1 hour at room temperature. It was stained with anti-Nanog antibody overnight at 4 degrees (right), and DAPI (left).

    See Abreview

  • Overlay histogram of Nanog staining in mouse embryonic stem cells using ab80892, at 1:100 dilution.

    Purple histogram represents negative control (rabbit IgG).

    Green line represents anti-Nanog antibody (ab80892).

    See Abreview

References

This product has been referenced in:
  • Pei Y  et al. Murine pluripotent stem cells that escape differentiation inside teratomas maintain pluripotency. PeerJ 6:e4177 (2018). Read more (PubMed: 29312817) »
  • Yu Y  et al. ERK inhibition promotes neuroectodermal precursor commitment by blocking self-renewal and primitive streak formation of the epiblast. Stem Cell Res Ther 9:2 (2018). Read more (PubMed: 29304842) »
See all 173 Publications for this product

Customer reviews and Q&As

1-10 of 27 Abreviews or Q&A

Application
Immunocytochemistry
Sample
Rabbit Cultured Cells (brain)
Permeabilization
Yes - tritonx100
Specification
brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Fixative
Paraformaldehyde

Kyungjoo Seong

Verified customer

Submitted Oct 31 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization
No
Specification
lung
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: 25°C
Fixative
10% Formalin

Abcam user community

Verified customer

Submitted Apr 25 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Embryonic)
Permeabilization
Yes - Triton-X100 (0.5%)
Specification
Embryonic
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 23 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Tissue lysate - whole (mouse cerebellum)
Specification
mouse cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 18 2014

Application
Flow Cytometry
Fixation
Paraformaldehyde
Permeabilization
Yes - 0.5% v/v tween20 in PBS
Sample
Mouse Cell (mouse embryonic stem cell)
Specification
mouse embryonic stem cell
Gating Strategy
live cells
Preparation
Cell harvesting/tissue preparation method: cell dissociation buffer
Sample buffer: 0.5% v/v tween20 in PBS

Abcam user community

Verified customer

Submitted Apr 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (lung)
Specification
lung
Fixative
Formaldehyde
Permeabilization
Yes - with Tris and Tween
Blocking step
Peroxidase as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 15 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: TRIS-EDTA Buffer pH 9,0
Permeabilization
Yes - Wash Buffer from Dako with Tween
Specification
Tonsil
Fixative
Paraformaldehyde

Mr. Rudolf Jung

Verified customer

Submitted Mar 22 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Common marmoset Tissue sections (embryonic testis E92)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Permeabilization
No
Specification
embryonic testis E92
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde

Dr. 煜欽 林

Verified customer

Submitted Feb 11 2013

Answer

DISCOUNT CODE: ABTRIAL-EBAPI
Ablaufdatum: 27/11/12

Die Frau Hoffmann ist zurzeit leider außer Haus, daher habe ich den AbTrial Code für Sie ausgestellt.

Es freut mich zu hören, dass Sie unser Angebot unseren Antikörper ab80892 in Hühnchen zu testen, annehmen möchten. Wie mit Frau Hoffmann besprochen, können Sie den Gutscheincode zum Kauf eines weiteren Antikörpers einsetzen, sobald Sie uns ein Abreview für diesen Antikörper eingereicht haben. Der Discountcode hat die Nummer ABTRIAL-EBAPI, deckt den Wert von €420.00 ab und kann zum Kauf weiterer Produkte eingesetzt werden.

Der Code wird gültig, sobald Sie uns ein Abreview für diesen Antikörper über den Test in Hühnchen eingereicht haben. Bitte geben Sie diesen Code auch im Abschnitt “Additional Notes” des Abreviews mit an, so dass wir wissen, dass sich dieses Abreview auf die Gutscheinaktion bezieht. Der Code wird dadurch aktiviert. Bitte kontaktieren Sie bei der nächsten Bestellung unsere Kundendienst mit dem Code und der ursprünglichen Bestellnummer.

Bitte zögern Sie nicht, sich wieder an uns zu wenden, falls Sie weitere Fragen haben. Wir freuen uns auf Ihr Abreview, egal ob Ihre Ergebnisse positiv oder negativ sind und wünschen Ihnen viel Glück für Ihre Forschung.

Die Bedingungen dieses Angebotes befinden sich unter dem folgenden Link: www.abcam.com/collaborationdiscount

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Answer

Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

Ich freue mich Ihnen mitteilen zu können, dass ab80892 (Anti-Nanog antibody) der selbe Antikörper ist wie ab21603. Bei ab80892 handelt um eine neue Charge von ab21603.
Ich entschuldige mich für die Verwirrung.

https://www.abcam.com/index.html?datasheet=80892 (or use the following: https://www.abcam.com/index.html?datasheet=80892).


Unseres Wissens haben wir noch keine Abbildung von ab80892 in Hühnchen.


Falls Sie uns eine Abbildung Ihrer Ergebnisse mit ab80892 zukommen lassen, kann ich Ihnen zurzeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat und einer Abbildung zusenden. Der Rabatt würde den ganzen Wert von ab80892 abdecken, und gegen eine erneute Bestellung ein weiterer Produkte von uns verrechnet werden.

Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

1.) Bestätigen Sie mir bitte, dass Sie ab80892 kaufen möchten und bereit sind uns eine Abbildung Ihrer Ergebnisse zukommen zu lassen.

2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

4.) Testen Sie den Antikörper in Hühnchen.

5.) Senden Sie uns die Abbildung Ihres Ergebnisses mittels eines Abreviews zu und notieren Sie den Discount Code in dem Feld "additional notes"
Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
https://www.abcam.com/abreviews

6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv, und Sie können weiter Produkte zu dem gleichen Preis wie ab80892 bei uns bestellen (halten Sie bei der Bestellung bitte den Rabattcode). Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
https://www.abcam.com/collaborationdiscount

Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

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1-10 of 27 Abreviews or Q&A

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