Anti-Nanog antibody (ab21624)
Key features and details
- Rabbit polyclonal to Nanog
- Suitable for: ICC/IF, WB, Sandwich ELISA
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Nanog antibody
See all Nanog primary antibodies -
Description
Rabbit polyclonal to Nanog -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, Sandwich ELISAmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Nanog aa 29-49.
Sequence:CGPEENYPSLQMSSAEMPHTE
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General notes
Concentration of Anti-Nanog antibody: 0.2 mg/mL
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab21624 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (10) |
1/1000.
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WB | (3) |
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa).
Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
Sandwich ELISA |
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture.
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Notes |
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ICC/IF
1/1000. |
WB
1/200. Detects a band of approximately 38 kDa (predicted molecular weight: 34 kDa). Some users report a stronger signal after blocking and diluting antibodies with 1% BSA instead of 5% milk. Mouse target cannot be detected by WB. |
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [1E6C4] to Nanog (ab76586) as Capture. |
Target
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Function
Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation. -
Tissue specificity
Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes. -
Sequence similarities
Belongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain. -
Developmental stage
Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 100293888 Human
- Entrez Gene: 79923 Human
- Omim: 607937 Human
- SwissProt: Q9H9S0 Human
- Unigene: 635882 Human
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Alternative names
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
see all
Images
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ab21624 at a 1/1000 dilution staining Nanog in human embryonic stem cells by ICC. An antibody towards Oct4, which has an identical staining pattern to ab21624, was used as a positive control.
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All lanes : Anti-Nanog antibody (ab21624) at 1/200 dilution
Lane 1 : Human fetal fibroblast lysate
Lane 2 : Human iPS cell lysate
Predicted band size: 34 kDa -
All lanes : Anti-Nanog antibody (ab21624) at 1/1000 dilution
Lane 1 : whole cell extract of mouse 3T3 cells expressing mouse Nanog.
Lane 2 : whole cell extract of mouse 3T3 cells expressing human Nanog.
Predicted band size: 34 kDa
Observed band size: 38 kDa why is the actual band size different from the predicted?
ab21624 recognizes human Nanog but does not recognize mouse Nanog in Western Blot. -
Immunocytochemical analysis of Human iPSCs labeling Nanog with ab21624 at 1/75 dilution.
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Standard Curve for Nanog (Analyte: Nanog protein (ab50053)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [1E6C4] to Nanog (ab76586) at 0.2 ug/ml and Detector Antibody Rabbit polyclonal to Nanog - ChIP Grade (ab21624) at 0.5 ug/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (257)
ab21624 has been referenced in 257 publications.
- Carrasco M et al. Spatial Environment Affects HNF4A Mutation-Specific Proteome Signatures and Cellular Morphology in hiPSC-Derived β-Like Cells. Diabetes 71:862-869 (2022). PubMed: 35043148
- Lopez-Fabuel I et al. Aberrant upregulation of the glycolytic enzyme PFKFB3 in CLN7 neuronal ceroid lipofuscinosis. Nat Commun 13:536 (2022). PubMed: 35087090
- Akter M et al. Generation of highly pure motor neurons from human induced pluripotent stem cells. STAR Protoc 3:101223 (2022). PubMed: 35300000
- Kameda K et al. Protocol for enhanced proliferation of human pluripotent stem cells in tryptophan-fortified media. STAR Protoc 3:101341 (2022). PubMed: 35496810
- Cloutier M et al. Preventing erosion of X-chromosome inactivation in human embryonic stem cells. Nat Commun 13:2516 (2022). PubMed: 35523820