Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (liver cancer)
Specification
liver cancer
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton-100
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C

Other product details

Dilution
1/500
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 647 donkey anti-rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Dilution
1/1000

Additional data

Additional Notes
Incubation at 4°C is good for nuclear localization.

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Submitted Oct 16 2012

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