Western blot abreview for Anti-Nanog antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (liver cancer)
Loading amount
10 µg
Specification
liver cancer
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: primary antibody dilution solution

Secondary antibody

Name
Non-Abcam antibody was used: HRP conjugated goat anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
SuperSignal West Dura
Exposure
30 second(s)
Bands
Specific: 42 kDa

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Submitted Oct 11 2012

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