Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Mouse ES cells exogenously expressing Human Nanog)
Specification
Mouse ES cells exogenously expressing Human Nanog
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% triton-X
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 16°C

Other product details

Dilution
1/100
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: block

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-rabbit IgG 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
WT D3 mouse ES cells 24 hours after transfection with a human Nanog encoding plasmid. The antibody has detected human Nanog alone expressed by only a small portion of the cells, and does not appear to cross-react with mouse Nanog at all. Blue staining is DAPI, green is ab21624.

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Submitted Oct 22 2012

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