Immunocytochemistry/ Immunofluorescence abreview for Anti-Nanog antibody - ChIP Grade

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iPSCs)
Permeabilization
Yes - 0.2% Triton for 10 min
Specification
iPSCs
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 5% Normal Goat Serum in PBS
Dilution
1/75

Secondary antibody

Dilution
1/1000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Secondary Antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
Image stained with Nanog (488) and SSEA4 (594) at 60X using widefield fluorescence microscope.

Mr. Mick Wauchope

Verified customer

Submitted Mar 16 2016

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