• Product name

    Anti-Nanog antibody [NNG-811]
    See all Nanog primary antibodies
  • Description

    Mouse monoclonal [NNG-811] to Nanog
  • Host species

  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, IP, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length human Nanog.

  • Positive control

    • Extracts of NT2 cells.



Our Abpromise guarantee covers the use of ab62734 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 35 kDa).
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.


  • Function

    Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
  • Tissue specificity

    Expressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
  • Sequence similarities

    Belongs to the Nanog homeobox family.
    Contains 1 homeobox DNA-binding domain.
  • Developmental stage

    Expressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • Embryonic stem cell specific homeobox protein (Nanog) antibody
    • ENK antibody
    • FLJ12581 antibody
    • hNanog antibody
    • Homeobox protein NANOG antibody
    • Homeobox transcription factor Nanog antibody
    • homeobox transcription factor Nanog-delta 48 antibody
    • NANOG antibody
    • Nanog homeobox antibody
    • NANOG_HUMAN antibody
    see all


  • 3T3 mouse fibroblasts were transfected with a mammalian expression vector expressing human Nanog and stained with ab62734 (5 mg/mL) followed by Goat Anti-Mouse, Cy3 conjugate. A clear detection of Nanog can be seen in nuclei of transfected cells.
  • Immunohistochemical analysis of PFA-fixed paraffin-embedded human neuronal tissue sections labelling Nanog with ab62734 at 1/100 incubated for 12 hours at 4°C. Heat mediated antigen retrival was with Citric buffer at pH6.0. Blocking was with 10% serum incubated for 1 hour at 20°C. Secondary used was a rabbit anti-mouse HRP conjugate at 1/200.

    See Abreview

  • Lane 1 : Anti-Nanog antibody [NNG-811] (ab62734) at 10 µg/ml
    Lane 2 : Anti-Nanog antibody [NNG-811] (ab62734) at 2 µg/ml
    Lane 3 : Negative control – no primary antibody

    All lanes : Cell extract of NT2 cells

    Lysates/proteins at 20 µg per lane.

    All lanes : Goat Anti-Mouse IgG

    Developed using the ECL technique.

    Predicted band size: 35 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?

  • Nanog was immunoprecipitated from NT2/D1 (human embryonal testis carcinoma cell line) whole cell extract using ab62734.

    Lane 1: 5 μg ab62734 IP in NT2/D1 whole cell extract.
    Lane 2: 10 μg ab62734 IP in NT2/D1 whole cell extract.
    Lane 3: Negative control without cell extract.
    Lane 4: NT2/D1 whole cell extract (input).

  • Paraffin-embedded human glioblastoma xenograft in nude mice stained for Nanog using ab62734 at 1/250 dilution in immunohistochemical analysis.


This product has been referenced in:

  • Meng TG  et al. Mettl14 is required for mouse postimplantation development by facilitating epiblast maturation. FASEB J 33:1179-1187 (2019). Read more (PubMed: 30110177) »
  • Chen S  et al. RER1 enhances carcinogenesis and stemness of pancreatic cancer under hypoxic environment. J Exp Clin Cancer Res 38:15 (2019). Read more (PubMed: 30630537) »
See all 29 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (human glioblastoma xenograft in nude mice)
human glioblastoma xenograft in nude mice
Alcool-Formol-Acetate (AFA)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA pH 9
Yes - Triton 0.1%
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C

Abcam user community

Verified customer

Submitted May 07 2013

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jun 08 2011

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