Key features and details
- Rat monoclonal [SER211] to Nanog
- Suitable for: WB
- Reacts with: Mouse
Product nameAnti-Nanog antibody [SER211]
See all Nanog primary antibodies
DescriptionRat monoclonal [SER211] to Nanog
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse
- WB: F9 whole cell lysate.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at +4°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 0.05% BSA, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab241542 in the following tested applications.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).|
FunctionTranscription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes (By similarity). Acts as a transcriptional activator or repressor (By similarity). Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' (By similarity). When overexpressed, promotes cells to enter into S phase and proliferation.
Tissue specificityExpressed in testicular carcinoma and derived germ cell tumors (at protein level). Expressed in fetal gonads, ovary and testis. Also expressed in ovary teratocarcinoma cell line and testicular embryonic carcinoma. Not expressed in many somatic organs and oocytes.
Sequence similaritiesBelongs to the Nanog homeobox family.
Contains 1 homeobox DNA-binding domain.
Developmental stageExpressed in embryonic stem (ES) and carcinoma (EC) cells. Expressed in inner cell mass (ICM) of the blastocyst and gonocytes between 14 and 19 weeks of gestation (at protein level). Not expressed in oocytes, unfertilized oocytes, 2-16 cell embryos and early morula (at protein level). Expressed in embryonic stem cells (ES). Expression decreases with ES differentiation.
- Information by UniProt
- Embryonic stem cell specific homeobox protein (Nanog) antibody
- ENK antibody
- FLJ12581 antibody
All lanes : Anti-Nanog antibody [SER211] (ab241542) at 1 µg/ml
Lane 1 : F9 whole cell lysate
Lane 2 : MEF1 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 34 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Lanes 1 - 4: Merged signal (red and green). Green - ab241542 observed at 40 kDa. Red - loading control, ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) observed at 55 kDa.
ab241542 was shown to react with Nanog in whole cell lysates subjected to SDS-PAGE. Membranes were blocked in 3% Milk in TBS-T (0.1% Tween®) before incubation with ab241542 and ab52866 (Anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 µg/µl and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
ab241542 has not yet been referenced specifically in any publications.