Overview

  • Product name

    Anti-NANOGP8 antibody
  • Description

    Rabbit polyclonal to NANOGP8
  • Host species

    Rabbit
  • Specificity

    ab74096 detects endogenous levels of total NANOGP8 protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal region of human NANOGP8.

  • Positive control

    • Extracts from Jurkat, HUVEC, HT-29 and HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74096 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 35 kDa.
ELISA 1/10000.

Target

  • Relevance

    NANOGP8 may act as a transcription regulator. When overexpressed, promotes cells to enter into S phase and proliferation. Expressed in tumor uterine cervix, breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines.
  • Cellular localization

    Nuclear
  • Database links

  • Alternative names

    • NANOG antibody
    • Nanog homeobox pseudogene 8 antibody
    • NANOGP1 antibody
    • NANOGP8 antibody

Images

  • All lanes : Anti-NANOGP8 antibody (ab74096) at 1/500 dilution

    Lane 1 : Extracts from Jurkat cells with no peptide
    Lane 2 : Extracts from HUVEC cells with no peptide
    Lane 3 : Extracts from HT-29 cells with no peptide
    Lane 4 : Extracts from HepG2 cells with no peptide
    Lane 5 : Extracts from Jurkat cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 35 kDa
    Observed band size: 35 kDa
    Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Jiang Z  et al. Oncogenic NanogP8 expression regulates cell proliferation and migration through the Akt/mTOR signaling pathway in human gastric cancer - SGC-7901cell line. Onco Targets Ther 9:4859-66 (2016). Read more (PubMed: 27563247) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your patience. The lab told me that they didn't test the antibody to check reactivity with Nanog. Thus, we do not have any data to prove that the antibody will not crossreact with Nanog.   Due to the high homology between both proteins it might be difficult to make an antibody that is exclusively specific to Nanogp8. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. 

Read More

Answer

Thank you for contacting us. I was in touch with the originating lab, and received the following information: "This antibody is specific to NANOGP8. The sequence homology is 92% with NANOG." I have asked them again if they have done any tests to exclude crossreactivity with Nanog since a 92% homology is quite high. I will let you know what I hear back. Thank you for your patience. In the meantime, please do not hesitate to contact us if you need any more advice or information.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up