Product nameAnti-NANOGP8 antibody
DescriptionRabbit polyclonal to NANOGP8
Specificityab74096 detects endogenous levels of total NANOGP8 protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthesized peptide derived from an internal region of human NANOGP8.
- Extracts from Jurkat, HUVEC, HT-29 and HepG2 cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab74096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 35 kDa.|
RelevanceNANOGP8 may act as a transcription regulator. When overexpressed, promotes cells to enter into S phase and proliferation. Expressed in tumor uterine cervix, breast and urinary bladder tissues, and also osteosarcoma, hepatoma, and breast adenocarcinoma cancer cell lines.
- NANOG antibody
- Nanog homeobox pseudogene 8 antibody
- NANOGP1 antibody
- NANOGP8 antibody
All lanes : Anti-NANOGP8 antibody (ab74096) at 1/500 dilution
Lane 1 : Extracts from Jurkat cells with no peptide
Lane 2 : Extracts from HUVEC cells with no peptide
Lane 3 : Extracts from HT-29 cells with no peptide
Lane 4 : Extracts from HepG2 cells with no peptide
Lane 5 : Extracts from Jurkat cells with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa
Additional bands at: 22 kDa. We are unsure as to the identity of these extra bands.
This product has been referenced in:
- Jiang Z et al. Oncogenic NanogP8 expression regulates cell proliferation and migration through the Akt/mTOR signaling pathway in human gastric cancer - SGC-7901cell line. Onco Targets Ther 9:4859-66 (2016). Read more (PubMed: 27563247) »