Overview

  • Product name

    Anti-NAP1L1 antibody [EPR11845]
    See all NAP1L1 primary antibodies
  • Description

    Rabbit monoclonal [EPR11845] to NAP1L1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAP1L1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P55209

  • Positive control

    • HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899). Human kidney tissue. HeLa cells. Permeabilized 293T cells. Immunoprecipitation pellet from 293T cell lysate.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab178687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 45 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

IP 1/10 - 1/100.

Target

  • Function

    May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
  • Tissue specificity

    Ubiquitously expressed.
  • Sequence similarities

    Belongs to the nucleosome assembly protein (NAP) family.
  • Domain

    The acidic domains are probably involved in the interaction with histones.
  • Post-translational
    modifications

    Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
  • Cellular localization

    Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ16112 antibody
    • hNRP antibody
    • HSP22 like protein interacting protein antibody
    • MGC23410 antibody
    • MGC8688 antibody
    • NAP 1 antibody
    • NAP 1 related protein antibody
    • NAP 1L antibody
    • NAP-1-related protein antibody
    • NAP1 antibody
    • NAP1 L1 antibody
    • NAP1 related protein antibody
    • NAP1L antibody
    • Nap1l1 antibody
    • NAP1L1 protein antibody
    • NP1L1_HUMAN antibody
    • NRP antibody
    • Nucleosome assembly protein 1 like 1 antibody
    • Nucleosome assembly protein 1-like 1 antibody
    • Nucleosome assembly protein I-related protein antibody
    see all

Images

  • All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution

    Lane 1 : HeLa lysate
    Lane 2 : 293T lysate
    Lane 3 : A431 lysate
    Lane 4 : Jurkat lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.

  • Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).

  • Flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

References

ab178687 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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