Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11845] to NAP1L1
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
- Reacts with: Human
Product nameAnti-NAP1L1 antibody [EPR11845]
See all NAP1L1 primary antibodies
DescriptionRabbit monoclonal [EPR11845] to NAP1L1
Tested applicationsSuitable for: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details
Species reactivityReacts with: Human
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human NAP1L1 aa 300 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P55209
- HeLa, 293T, A431 and Jurkat whole cell lysate (ab7899). Human kidney tissue. HeLa cells. Permeabilized 293T cells. Immunoprecipitation pellet from 293T cell lysate.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab178687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 45 kDa.|
|ICC/IF||1/100 - 1/250.|
|IHC-P||1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|IP||1/10 - 1/100.|
FunctionMay be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
DomainThe acidic domains are probably involved in the interaction with histones.
modificationsPolyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
Cellular localizationNucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- FLJ16112 antibody
- hNRP antibody
- HSP22 like protein interacting protein antibody
All lanes : Anti-NAP1L1 antibody [EPR11845] (ab178687) at 1/10000 dilution
Lane 1 : HeLa lysate
Lane 2 : 293T lysate
Lane 3 : A431 lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 45 kDa
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAP1L1 with ab178687 at a 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescence analysis of HeLa cells labeling NAP1L1 with ab178687 at a 1/100 dilution. DAPI nuclear staining (blue).
Flow cytometric analysis of permeabilized 293T cells using ab178687 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
Western blot analysis on immunoprecipitation pellet from (Lane 1) 293T cell lysate or (Lane 2) 1X PBS (negative control) using ab178687 at a 1/10 dilution for IP and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
ab178687 has not yet been referenced specifically in any publications.