Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR12619] to NAP1L1
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-NAP1L1 antibody [EPR12619]
See all NAP1L1 primary antibodies
DescriptionRabbit monoclonal [EPR12619] to NAP1L1
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human NAP1L1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: P55209
- HeLa, Jurkat, 293T and A431 cell lysates; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 52-60 kDa (predicted molecular weight: 45 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionMay be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the nucleosome assembly protein (NAP) family.
DomainThe acidic domains are probably involved in the interaction with histones.
modificationsPolyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
Cellular localizationNucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- FLJ16112 antibody
- hNRP antibody
- HSP22 like protein interacting protein antibody
All lanes : Anti-NAP1L1 antibody [EPR12619] (ab174836) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : 293T cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 45 kDa
Flow cytometric analysis of permeabilized Jurkat cells labeling NAP1L1with ab174836 at 1/10 dilution (red), or negative control rabbit IgG (green).
ab174836 has not yet been referenced specifically in any publications.