Overview

  • Product name
    Anti-NAP1L1 antibody [EPR12619]
    See all NAP1L1 primary antibodies
  • Description
    Rabbit monoclonal [EPR12619] to NAP1L1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human NAP1L1 aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: P55209

  • Positive control
    • HeLa, Jurkat, 293T and A431 cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174836 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 52-60 kDa (predicted molecular weight: 45 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      May be involved in modulating chromatin formation and contribute to regulation of cell proliferation.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the nucleosome assembly protein (NAP) family.
    • Domain
      The acidic domains are probably involved in the interaction with histones.
    • Post-translational
      modifications
      Polyglutamylated by TTLL4, a modification that occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Some residues may also be monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human.
    • Cellular localization
      Nucleus. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • FLJ16112 antibody
      • hNRP antibody
      • HSP22 like protein interacting protein antibody
      • MGC23410 antibody
      • MGC8688 antibody
      • NAP 1 antibody
      • NAP 1 related protein antibody
      • NAP 1L antibody
      • NAP-1-related protein antibody
      • NAP1 antibody
      • NAP1 L1 antibody
      • NAP1 related protein antibody
      • NAP1L antibody
      • Nap1l1 antibody
      • NAP1L1 protein antibody
      • NP1L1_HUMAN antibody
      • NRP antibody
      • Nucleosome assembly protein 1 like 1 antibody
      • Nucleosome assembly protein 1-like 1 antibody
      • Nucleosome assembly protein I-related protein antibody
      see all

    Images

    • All lanes : Anti-NAP1L1 antibody [EPR12619] (ab174836) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : Jurkat cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 45 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling NAP1L1with ab174836 at 1/10 dilution (red), or negative control rabbit IgG (green).

    References

    ab174836 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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