Anti-NAPE-PLD antibody (ab246951)
Key features and details
- Rabbit polyclonal to NAPE-PLD
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-NAPE-PLD antibody
See all NAPE-PLD primary antibodies -
Description
Rabbit polyclonal to NAPE-PLD -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human NAPE-PLD aa 100-200.
Database link: Q6IQ20 -
Positive control
- IHC-P: Human kidney tissue. ICC/IF: A431 cells. WB: Human liver tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab246951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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|
WB |
Use a concentration of 0.04 - 0.4 µg/ml.
|
Notes |
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ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Function
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. -
Sequence similarities
Belongs to the NAPE-PLD family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 222236 Human
- Omim: 612334 Human
- SwissProt: Q6IQ20 Human
- Unigene: 324271 Human
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Alternative names
- C7orf18 antibody
- DKFZp781D1098 antibody
- FMP30 antibody
see all
Images
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FA fixed, Triton X-100 permeabilized A431 (Human epidermoid carcinoma cell line) cells labeling NAPE-PLD using ab246951 at 4 µg/ml (green) in ICC/IF.
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Paraffin-embedded human kidney tissue stained for NAPE-PLD using ab246951 at 1/50 dilution in immunohistochemical analysis.
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All lanes : Anti-NAPE-PLD antibody (ab246951) at 0.4 µg/ml
Lane 1 : RT4 (Human urinary bladder cancer cell line) cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab246951 has been referenced in 1 publication.
- Duncan RS et al. N-acylethanolamide metabolizing enzymes are upregulated in human neural progenitor-derived neurons exposed to sub-lethal oxidative stress. Front Cell Neurosci 16:902278 (2022). PubMed: 36003139