Anti-NAPE-PLD antibody (ab95397)
Key features and details
- Rabbit polyclonal to NAPE-PLD
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NAPE-PLD antibody
See all NAPE-PLD primary antibodies -
Description
Rabbit polyclonal to NAPE-PLD -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Hamster, Orangutan
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Immunogen
Synthetic peptide:
YMGPKRFRRSPCTI
, corresponding to amino acids 159 - 172 of Human NAPE-PLD (UniProt: Q6IQ20). -
Positive control
- Human cerebellum supernatant, Mouse brain homogenate and Mouse brain high density membrane
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/200. Predicted molecular weight: 46 kDa.
|
| Notes |
|---|
|
WB
1/200. Predicted molecular weight: 46 kDa. |
Target
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Function
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. -
Sequence similarities
Belongs to the NAPE-PLD family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 222236 Human
- Entrez Gene: 242864 Mouse
- Omim: 612334 Human
- SwissProt: Q6IQ20 Human
- SwissProt: Q8BH82 Mouse
- Unigene: 324271 Human
- Unigene: 153292 Mouse
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Alternative names
- C7orf18 antibody
- DKFZp781D1098 antibody
- FMP30 antibody
see all
Images
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Western blot - Anti-NAPE-PLD antibody (ab95397)All lanes : Anti-NAPE-PLD antibody (ab95397) at 3 µg/ml
Lane 1 : Human cerebellum supernatant at 20 µg
Lane 2 : Mouse brain homogenate at 30 µg
Lane 3 : Mouse brain high density membrane at 30 µg
Predicted band size: 46 kDa
Datasheets and documents
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Datasheet download
References (11)
ab95397 has been referenced in 11 publications.
- Soares PN et al. Late effects of early weaning on food preference and the dopaminergic and endocannabinoid systems in male and female rats. J Dev Orig Health Dis N/A:1-11 (2021). PubMed: 33650480
- Rivera P et al. Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model. Nutrients 12:N/A (2020). PubMed: 32575416
- Mock ED et al. Discovery of a NAPE-PLD inhibitor that modulates emotional behavior in mice. Nat Chem Biol 16:667-675 (2020). PubMed: 32393901
- Xue SS et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
- Li MD et al. Adipocyte OGT governs diet-induced hyperphagia and obesity. Nat Commun 9:5103 (2018). PubMed: 30504766
