Anti-NAPE-PLD antibody (ab95397)
Key features and details
- Rabbit polyclonal to NAPE-PLD
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-NAPE-PLD antibody
See all NAPE-PLD primary antibodies -
Description
Rabbit polyclonal to NAPE-PLD -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
YMGPKRFRRSPCTI
, corresponding to amino acids 159 - 172 of Human NAPE-PLD (UniProt: Q6IQ20). -
Positive control
- Human cerebellum supernatant, Mouse brain homogenate and Mouse brain high density membrane
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95397 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Guaranteed
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted
Predicted to work for this combination of applications and species but not guaranteed.
Incompatible
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| All applications |
Hamster
Orangutan
|
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/200. Predicted molecular weight: 46 kDa.
|
| Notes |
|---|
|
WB
1/200. Predicted molecular weight: 46 kDa. |
Target
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Function
Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors. -
Sequence similarities
Belongs to the NAPE-PLD family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 222236 Human
- Entrez Gene: 242864 Mouse
- Omim: 612334 Human
- SwissProt: Q6IQ20 Human
- SwissProt: Q8BH82 Mouse
- Unigene: 324271 Human
- Unigene: 153292 Mouse
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Alternative names
- C7orf18 antibody
- DKFZp781D1098 antibody
- FMP30 antibody
see all
Images
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Western blot - Anti-NAPE-PLD antibody (ab95397)All lanes : Anti-NAPE-PLD antibody (ab95397) at 3 µg/ml
Lane 1 : Human cerebellum supernatant at 20 µg
Lane 2 : Mouse brain homogenate at 30 µg
Lane 3 : Mouse brain high density membrane at 30 µg
Predicted band size: 46 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (9)
ab95397 has been referenced in 9 publications.
- Rivera P et al. Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model. Nutrients 12:N/A (2020). PubMed: 32575416
- Xue SS et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
- Li MD et al. Adipocyte OGT governs diet-induced hyperphagia and obesity. Nat Commun 9:5103 (2018). PubMed: 30504766
- Smaga I et al. Changes in the Brain Endocannabinoid System in Rat Models of Depression. Neurotox Res 31:421-435 (2017). PubMed: 28247204
- Martin GG et al. Fabp-1 gene ablation impacts brain endocannabinoid system in male mice. J Neurochem : (2016). PubMed: 27167970
- Martin GG et al. Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels. Lipids N/A:N/A (2016). WB ; Mouse . PubMed: 27450559
- Geurts L et al. Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota. Nat Commun 6:6495 (2015). WB ; Mouse . PubMed: 25757720
- Kaczocha M et al. Fatty acid binding protein deletion suppresses inflammatory pain through endocannabinoid/N-acylethanolamine-dependent mechanisms. Mol Pain 11:52 (2015). WB ; Mouse . PubMed: 26311517
- Pavón FJ et al. Effects of Adolescent Intermittent Alcohol Exposure on the Expression of Endocannabinoid Signaling-Related Proteins in the Spleen of Young Adult Rats. PLoS One 11:e0163752 (0). WB . PubMed: 27662369