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Signal Transduction Signaling Pathway Lipid Signaling Other
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Anti-NAPE-PLD antibody (ab95397)

  • Datasheet
  • SDS
Reviews (1) Submit a question References (9)

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Western blot - Anti-NAPE-PLD antibody (ab95397)

    Key features and details

    • Rabbit polyclonal to NAPE-PLD
    • Suitable for: WB
    • Reacts with: Mouse, Human
    • Isotype: IgG

    You may also be interested in

    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-NAPE-PLD antibody
      See all NAPE-PLD primary antibodies
    • Description

      Rabbit polyclonal to NAPE-PLD
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      YMGPKRFRRSPCTI

      , corresponding to amino acids 159 - 172 of Human NAPE-PLD (UniProt: Q6IQ20).
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • Positive control

      • Human cerebellum supernatant, Mouse brain homogenate and Mouse brain high density membrane

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: 0.1% BSA, 50% Glycerol, Tris buffered saline
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Other
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab95397 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    All applications
    Hamster
    Orangutan
    Application Abreviews Notes
    WB (1)
    1/200. Predicted molecular weight: 46 kDa.
    Notes
    WB
    1/200. Predicted molecular weight: 46 kDa.

    Target

    • Function

      Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors.
    • Sequence similarities

      Belongs to the NAPE-PLD family.
    • Cellular localization

      Membrane.
    • Target information above from: UniProt accession Q6IQ20 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 222236 Human
      • Entrez Gene: 242864 Mouse
      • Omim: 612334 Human
      • SwissProt: Q6IQ20 Human
      • SwissProt: Q8BH82 Mouse
      • Unigene: 324271 Human
      • Unigene: 153292 Mouse
      • Alternative names

        • C7orf18 antibody
        • DKFZp781D1098 antibody
        • FMP30 antibody
        • Mbldc1 antibody
        • N acyl phosphatidylethanolamine hydrolyzing phospholipase D antibody
        • N acyl phosphatidylethanolamine phospholipase D antibody
        • N-acyl phosphatidylethanolamine phospholipase D antibody
        • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D antibody
        • NAPE hydrolyzing phospholipase D antibody
        • NAPE-hydrolyzing phospholipase D antibody
        • NAPE-PLD antibody
        • NAPEP_HUMAN antibody
        • NAPEPLD antibody
        see all

      Images

      • Western blot - Anti-NAPE-PLD antibody (ab95397)
        Western blot - Anti-NAPE-PLD antibody (ab95397)
        All lanes : Anti-NAPE-PLD antibody (ab95397) at 3 µg/ml

        Lane 1 : Human cerebellum supernatant at 20 µg
        Lane 2 : Mouse brain homogenate at 30 µg
        Lane 3 : Mouse brain high density membrane at 30 µg

        Predicted band size: 46 kDa

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (9)

      Publishing research using ab95397? Please let us know so that we can cite the reference in this datasheet.

      ab95397 has been referenced in 9 publications.

      • Rivera P  et al. Sex-Specific Anxiety and Prefrontal Cortex Glutamatergic Dysregulation Are Long-Term Consequences of Pre-and Postnatal Exposure to Hypercaloric Diet in a Rat Model. Nutrients 12:N/A (2020). PubMed: 32575416
      • Xue SS  et al. Repetitive high-frequency transcranial magnetic stimulation reverses depressive-like behaviors and protein expression at hippocampal synapses in chronic unpredictable stress-treated rats by enhancing endocannabinoid signaling. Pharmacol Biochem Behav 184:172738 (2019). PubMed: 31229467
      • Li MD  et al. Adipocyte OGT governs diet-induced hyperphagia and obesity. Nat Commun 9:5103 (2018). PubMed: 30504766
      • Smaga I  et al. Changes in the Brain Endocannabinoid System in Rat Models of Depression. Neurotox Res 31:421-435 (2017). PubMed: 28247204
      • Martin GG  et al. Fabp-1 gene ablation impacts brain endocannabinoid system in male mice. J Neurochem : (2016). PubMed: 27167970
      • Martin GG  et al. Female Mice are Resistant to Fabp1 Gene Ablation-Induced Alterations in Brain Endocannabinoid Levels. Lipids N/A:N/A (2016). WB ; Mouse . PubMed: 27450559
      • Geurts L  et al. Adipose tissue NAPE-PLD controls fat mass development by altering the browning process and gut microbiota. Nat Commun 6:6495 (2015). WB ; Mouse . PubMed: 25757720
      • Kaczocha M  et al. Fatty acid binding protein deletion suppresses inflammatory pain through endocannabinoid/N-acylethanolamine-dependent mechanisms. Mol Pain 11:52 (2015). WB ; Mouse . PubMed: 26311517
      • Pavón FJ  et al. Effects of Adolescent Intermittent Alcohol Exposure on the Expression of Endocannabinoid Signaling-Related Proteins in the Spleen of Young Adult Rats. PLoS One 11:e0163752 (0). WB . PubMed: 27662369

      Customer reviews and Q&As

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      Western blot abreview for Anti-NAPE-PLD antibody

      Poor
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Dog Cell lysate - whole cell (melanoma cell line)
      Gel Running Conditions
      Reduced Denaturing
      Loading amount
      10 µg
      Specification
      melanoma cell line
      Blocking step
      (agent) for 50 minute(s) · Concentration: 100%
      Read More

      Dr. 令 中野

      Verified customer

      Submitted Jun 20 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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