Overview

  • Product name

    Anti-NAPE-PLD antibody [OTI3D5]
    See all NAPE-PLD primary antibodies
  • Description

    Mouse monoclonal [OTI3D5] to NAPE-PLD
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant full length protein corresponding to Human NAPE-PLD aa 1-393. Produced in HEK-293T cells (NP_075060).
    Database link: Q6IQ20

  • Positive control

    • WB: HEK-293T cell lysate transfected with pCMV6-ENTRY NAPE-PLD cDNA. IHC-P: Human endometrium adenocarcinoma, bladder carcinoma and tonsil tissue. Flow Cyt: SHSY-5Y cells.
  • General notes

    Clone OTI3D5 (formerly 3D5).

Properties

Applications

Our Abpromise guarantee covers the use of ab119259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 46 kDa.
IHC-P 1/150. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/1000.

ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Hydrolyzes N-acyl-phosphatidylethanolamines (NAPEs) to produce N-acylethanolamines (NAEs) and phosphatidic acid. Responsible for the generation of anandamide (N-arachidonoylethanolamine), the ligand of cannabinoid and vanilloid receptors.
  • Sequence similarities

    Belongs to the NAPE-PLD family.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • C7orf18 antibody
    • DKFZp781D1098 antibody
    • FMP30 antibody
    • Mbldc1 antibody
    • N acyl phosphatidylethanolamine hydrolyzing phospholipase D antibody
    • N acyl phosphatidylethanolamine phospholipase D antibody
    • N-acyl phosphatidylethanolamine phospholipase D antibody
    • N-acyl-phosphatidylethanolamine-hydrolyzing phospholipase D antibody
    • NAPE hydrolyzing phospholipase D antibody
    • NAPE-hydrolyzing phospholipase D antibody
    • NAPE-PLD antibody
    • NAPEP_HUMAN antibody
    • NAPEPLD antibody
    see all

Images

  • All lanes : Anti-NAPE-PLD antibody [OTI3D5] (ab119259) at 1/2000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control cDNA
    Lane 2 : HEK-293T cell lysate transfected with pCMV6-ENTRY NAPE-PLD cDNA

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 46 kDa

  • Overlay histogram showing SHSY-5Y (Human neuroblastoma cell line from bone marrow) cells stained with ab119259 (red line). The cells were fixed with 4% paraformaldehyde (10 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab119259, 1/1000 dilution) for 30 minutes at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 minutes at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 1μg/1x106 cells) used under the same conditions. Unlabeled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SHSY-5Y cells fixed with 80% methanol (5 minutes)/permeabilized with 0.1% PBS-Tween for 20 minutes used under the same conditions.

  • Paraffin-embedded human endometrium adenocarcinoma tissue stained for NAPE-PLD with ab119259 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Paraffin-embedded human bladder carcinoma tissue stained for NAPE-PLD with ab119259 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

  • Paraffin-embedded human tonsil tissue stained for NAPE-PLD with ab119259 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

References

ab119259 has not yet been referenced specifically in any publications.

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