Overview

  • Product name

  • Description

    Rabbit polyclonal to NAPRT1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 107-408 of Human NAPRT1.

  • Positive control

    • A549, HeLa, HepG2 and HCT116 whole cell lysates; Human lung carcinoma tissue, HeLa cells.
  • General notes

    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab127699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000 - 1/20000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells.
  • Pathway

    Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1.
  • Sequence similarities

    Belongs to the NAPRTase family.
  • Cellular localization

    Cytoplasm > cytosol.
  • Information by UniProt
  • Database links

  • Alternative names

    • FHA HIT interacting protein antibody
    • FHA-HIT-interacting protein antibody
    • FHIP antibody
    • NAPRT1 antibody
    • NAPRTase antibody
    • Nicotinate phosphoribosyltransferase antibody
    • Nicotinate phosphoribosyltransferase domain containing 1 antibody
    • Nicotinate phosphoribosyltransferase domain-containing protein 1 antibody
    • Nicotinic acid phosphoribosyltransferase antibody
    • PNCB_HUMAN antibody
    • PP3856 antibody
    see all

Images

  • Anti-NAPRT1 antibody (ab127699) at 1/10000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • ab127699, at a dilution of 1/500, staining NAPRT1 in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
  • ab127699, at a dilution of 1/500, staining NAPRT1 in methanol-fixed HeLa cells by Immunofluorescence (Green). Alpha-tubulin filaments were stained Red.

References

This product has been referenced in:

  • James EL  et al. Replicatively senescent human fibroblasts reveal a distinct intracellular metabolic profile with alterations in NAD+ and nicotinamide metabolism. Sci Rep 6:38489 (2016). Read more (PubMed: 27924925) »
See 1 Publication for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab127699.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up