Anti-NAPRT1 antibody [CL0665] (ab211529)
Key features and details
- Mouse monoclonal [CL0665] to NAPRT1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-NAPRT1 antibody [CL0665]
See all NAPRT1 primary antibodies -
Description
Mouse monoclonal [CL0665] to NAPRT1 -
Host species
Mouse -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human NAPRT1 aa 50-200.
Database link: Q6XQN6 -
Epitope
Binds to an epitope located within the peptide sequence PAFFEHLRALDCSEV (aa90-104) as determined by overlapping synthetic peptides. -
Positive control
- Human liver tissue lysate; Human kidney, stomach, placenta, testis, colorectal cancer and breast cancer tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0665 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab211529 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 58 kDa.
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Notes |
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IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 58 kDa. |
Target
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Function
Catalyzes the conversion of nicotinic acid (NA) to NA mononucleotide (NaMN). Essential for NA to increase cellular NAD levels and prevent oxidative stress of the cells. -
Pathway
Cofactor biosynthesis; NAD(+) biosynthesis; nicotinate D-ribonucleotide from nicotinate: step 1/1. -
Sequence similarities
Belongs to the NAPRTase family. -
Cellular localization
Cytoplasm > cytosol. - Information by UniProt
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Database links
- Entrez Gene: 399559 Cow
- Entrez Gene: 93100 Human
- Entrez Gene: 223646 Mouse
- Entrez Gene: 315085 Rat
- Omim: 611552 Human
- SwissProt: A5PK51 Cow
- SwissProt: Q6XQN6 Human
- SwissProt: Q8CC86 Mouse
see all -
Alternative names
- FHA HIT interacting protein antibody
- FHA-HIT-interacting protein antibody
- FHIP antibody
see all
Images
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Immunohistochemical analysis of paraffin-embedded Humanbreast cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAPRT1 with ab211529 at 1/200 dilution.
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Anti-NAPRT1 antibody [CL0665] (ab211529) at 1/500 dilution + Human liver tissue lysate
Predicted band size: 58 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab211529 has been referenced in 2 publications.
- Thongon N et al. Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA. Cancer Metab 6:1 (2018). PubMed: 29541451
- Piacente F et al. Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair. Cancer Res 77:3857-3869 (2017). PubMed: 28507103