Overview

  • Product name

    Anti-NAPRT1 antibody [CL0665]
    See all NAPRT1 primary antibodies
  • Description

    Mouse monoclonal [CL0665] to NAPRT1
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human NAPRT1 aa 90-161.
    Sequence:

    PAFFEHLRALDCSEVTVRALPEGSLAFPGVPLLQVSGPLLVVQLLETPLL CLVSYASLVATNAARLRLIAGP


    Database link: Q6XQN6

  • Epitope

    Binds to an epitope located within the peptide sequence PAFFEHLRALDCSEV (aa90-104) as determined by overlapping synthetic peptides.
  • Positive control

    • Human liver tissue lysate; Human kidney, stomach, placenta, testis, colorectal cancer and breast cancer tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab211529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 58 kDa.

Target

Images

  • Immunohistochemical analysis of paraffin-embedded Humanbreast cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human colorectal cancer tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling NAPRT1 with ab211529 at 1/200 dilution.

  • Anti-NAPRT1 antibody [CL0665] (ab211529) at 1/500 dilution + Human liver tissue lysate

    Predicted band size: 58 kDa

References

This product has been referenced in:

  • Thongon N  et al. Cancer cell metabolic plasticity allows resistance to NAMPT inhibition but invariably induces dependence on LDHA. Cancer Metab 6:1 (2018). Read more (PubMed: 29541451) »
  • Piacente F  et al. Nicotinic Acid Phosphoribosyltransferase Regulates Cancer Cell Metabolism, Susceptibility to NAMPT Inhibitors, and DNA Repair. Cancer Res 77:3857-3869 (2017). Read more (PubMed: 28507103) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Mouse Tissue lysate - whole (Kidney, Liver, Spleen, 4T1, OVCAR-5)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
20 µg
Specification
Kidney, Liver, Spleen, 4T1, OVCAR-5
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Francesco Piacente

Verified customer

Submitted Oct 04 2018

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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