Overview

  • Product name

    Anti-NAPSIN A antibody [EPR6252]
    See all NAPSIN A primary antibodies
  • Description

    Rabbit monoclonal [EPR6252] to NAPSIN A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human NAPSIN A aa 1-150. The exact sequence is proprietary.
    Database link: O96009

  • Positive control

    • A549 whole cell lysate (ab7910) can be used as a positive control in WB. Human lung adenocarcinoma lysate; Human lung adenocarcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133249 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 45 kDa.
IP 1/10 - 1/100.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      May be involved in processing of pneumocyte surfactant precursors.
    • Tissue specificity

      Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Asp 4 antibody
      • ASP4 antibody
      • Aspartyl protease 4 antibody
      • KAP antibody
      • Kdap antibody
      • Kidney derived aspartic protease like protein antibody
      • NAP1 antibody
      • NAPA antibody
      • Napsa antibody
      • NAPSA_HUMAN antibody
      • Napsin 1 antibody
      • napsin A aspartic peptidase antibody
      • Napsin A precursor antibody
      • Napsin-1 antibody
      • Napsin-A antibody
      • Pronapsin A antibody
      • SNAPA antibody
      • TA01/TA02 antibody
      see all

    Images

    • Anti-NAPSIN A antibody [EPR6252] (ab133249) at 1/10000 dilution + Human lung adenocarcinoma lysate at 10 µg

      Predicted band size: 45 kDa

    • Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling NAPSIN A with ab133249 at 1/250 dilution.
    • ab133249 showing positive staining in Normal lung tissue.

    • ab133249 showing negative staining in Lung squamous carcinoma tissue.

    • ab133249 showing positive staining in Normal kidney tissue.

    References

    This product has been referenced in:

    • Zhou L  et al. Overexpression of Napsin A resensitizes drug-resistant lung cancer A549 cells to gefitinib by inhibiting EMT. Oncol Lett 16:2533-2538 (2018). Read more (PubMed: 30008942) »
    • Lee HW  et al. Patient-derived xenografts from non-small cell lung cancer brain metastases are valuable translational platforms for the development of personalized targeted therapy. Clin Cancer Res 21:1172-82 (2015). Read more (PubMed: 25549722) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133249.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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