Recombinant Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
![Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-410668-anti-napsin-a-antibody-epr6252-bsa-and-azide-free-western-blot.jpg "Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6252] to NAPSIN A - BSA and Azide free
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Overview
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Product name
Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free
See all NAPSIN A primary antibodies -
Description
Rabbit monoclonal [EPR6252] to NAPSIN A - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, IPmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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General notes
ab248424 is the carrier-free version of ab133249 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248424 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6252 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab248424 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
|
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| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| IP |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa. |
|
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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IP
Use at an assay dependent concentration. |
Target
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Function
May be involved in processing of pneumocyte surfactant precursors. -
Tissue specificity
Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes. -
Sequence similarities
Belongs to the peptidase A1 family. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 9476 Human
- Omim: 605631 Human
- SwissProt: O96009 Human
- Unigene: 512843 Human
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Alternative names
- Asp 4 antibody
- ASP4 antibody
- Aspartyl protease 4 antibody
see all
Images
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Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-4-anti-napsin-a-antibody-epr6252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)This data was developed using ab133249, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling NAPSIN A with ab133249 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-3-anti-napsin-a-antibody-epr6252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-2-anti-napsin-a-antibody-epr6252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-1-anti-napsin-a-antibody-epr6252-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424) -
![Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)](https://www.abcam.com/ps/products/248/ab248424/Images/ab248424-6-benefits-of-recombinant-antibodies.png)
Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab248424 has not yet been referenced specifically in any publications.