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RecombinantRabMAb

Recombinant Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)

  • Datasheet
  • SDS
  • Certificate of Compliance
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Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
  • Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6252] to NAPSIN A - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

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Overview

  • Product name

    Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free
    See all NAPSIN A primary antibodies
  • Description

    Rabbit monoclonal [EPR6252] to NAPSIN A - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab248424 is the carrier-free version of ab133249 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab248424 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6252
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Other proteases

Associated products

  • Alternative Versions

    • Anti-NAPSIN A antibody [EPR6252] (ab133249)
  • Conjugation kits

    • FITC Conjugation Kit (Fast) - Lightning-Link® (ab188285)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control - BSA and Azide Free (ab210849)
  • Positive Controls

    • A549 whole cell lysate (ab7910)
  • Recombinant Protein

    • Recombinant Human NAPSIN A protein (denatured) (ab202144)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab248424 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
Notes
WB
Use at an assay dependent concentration. Predicted molecular weight: 45 kDa.
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      May be involved in processing of pneumocyte surfactant precursors.
    • Tissue specificity

      Expressed predominantly in adult lung (type II pneumocytes) and kidney and in fetal lung. Low levels in adult spleen and very low levels in peripheral blood leukocytes.
    • Sequence similarities

      Belongs to the peptidase A1 family.
    • Cellular localization

      Secreted.
    • Target information above from: UniProt accession O96009 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 9476 Human
      • Omim: 605631 Human
      • SwissProt: O96009 Human
      • Unigene: 512843 Human
      • Alternative names

        • Asp 4 antibody
        • ASP4 antibody
        • Aspartyl protease 4 antibody
        • KAP antibody
        • Kdap antibody
        • Kidney derived aspartic protease like protein antibody
        • NAP1 antibody
        • NAPA antibody
        • Napsa antibody
        • NAPSA_HUMAN antibody
        • Napsin 1 antibody
        • napsin A aspartic peptidase antibody
        • Napsin A precursor antibody
        • Napsin-1 antibody
        • Napsin-A antibody
        • Pronapsin A antibody
        • SNAPA antibody
        • TA01/TA02 antibody
        see all

      Images

      • Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Western blot - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Anti-NAPSIN A antibody [EPR6252] (ab133249) at 1/10000 dilution + Human lung adenocarcinoma tissue lysate at 10 µg

        Predicted band size: 45 kDa



        This data was developed using ab133249, the same antibody clone in a different buffer formulation.

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        This data was developed using ab133249, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue labelling NAPSIN A with ab133249 at 1/250 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        This data was developed using ab133249, the same antibody clone in a different buffer formulation.ab133249 showing positive staining in Normal lung tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        This data was developed using ab133249, the same antibody clone in a different buffer formulation.ab133249 showing negative staining in Lung squamous carcinoma tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        This data was developed using ab133249, the same antibody clone in a different buffer formulation.ab133249 showing positive staining in Normal kidney tissue.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
      • Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)
        Anti-NAPSIN A antibody [EPR6252] - BSA and Azide free (ab248424)

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      Certificate of Compliance

      To download a Certificate of Compliance, please enter your Lot number below:

      References (0)

      Publishing research using ab248424? Please let us know so that we can cite the reference in this datasheet.

      ab248424 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

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